Evidence map›Paper›PMID 37033870›Full record

ArticleACS omega2023

Synthesizing Submicron Polyelectrolyte Capsules to Boost Enzyme Immobilization and Enhance Enzyme-Based Immunoassays.

Yanna Shao, Yaofeng Zhou, Nuo Chen, Wenxing Xu, Huan Zhou, Weihua Lai, Xiaolin Huang, Xinran Xiang, Qinghua Ye, Jumei Zhang and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 2 countries.

Yanna ShaoDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Yaofeng ZhouCenter for Hybrid Nanostructures (CHyN), Universität Hamburg, 22607 Hamburg, Germany.
Nuo ChenDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Wenxing XuDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Huan ZhouDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.
Weihua LaiState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.ORCID https://orcid.org/0000-0002-9305-1522
Xiaolin HuangState Key Laboratory of Food Science and Technology, School of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Xinran XiangInstitute of Microbiology; State Key Laboratory of Applied Microbiology Southern China; Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Academy of Sciences, Guangzhou 510070, China.
Qinghua YeInstitute of Microbiology; State Key Laboratory of Applied Microbiology Southern China; Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Academy of Sciences, Guangzhou 510070, China.
Jumei ZhangInstitute of Microbiology; State Key Laboratory of Applied Microbiology Southern China; Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Academy of Sciences, Guangzhou 510070, China.
Juan WangCollege of Food Science, South China Agricultural University, Guangzhou 510432, China.
Wolfgang J ParakCenter for Hybrid Nanostructures (CHyN), Universität Hamburg, 22607 Hamburg, Germany.ORCID https://orcid.org/0000-0003-1672-6650
Qingping WuInstitute of Microbiology; State Key Laboratory of Applied Microbiology Southern China; Key Laboratory of Agricultural Microbiomics and Precision Application, Ministry of Agriculture and Rural Affairs; Guangdong Provincial Key Laboratory of Microbial Safety and Health, Guangdong Academy of Sciences, Guangzhou 510070, China.ORCID https://orcid.org/0000-0001-6503-359X
Yu DingDepartment of Food Science and Engineering, Institute of Food Safety and Nutrition, College of Science & Engineering, College of Life Science and Technology, Jinan University, Guangzhou 510632, China.ORCID https://orcid.org/0000-0002-3688-7294
Jinan University · CNGuangdong Academy of Sciences · CNNanchang University · CNSouth China Agricultural University · CNUniversität Hamburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polyelectrolyte capsules (PCs) exhibit attractive superiorities in enzyme immobilization, including providing a capacious microenvironment for enzyme conformational freedom, highly effective mass transfer, and protecting enzymes from the external environment. Herein, we provide the first systemic evaluation of submicron PCs (SPCs, 500 nm) for enzyme immobilization. The catalytic kinetics results show that SPC encapsulation affected the affinities of enzymes and substrates but significantly enhanced their catalytic activity. The stability test indicates that SPC-encapsulated horseradish peroxidase (HRP) exhibits ultrahigh resistance to external harsh conditions and has a longer storage life than that of soluble HRP. The proposed encapsulation strategy enables 7.73-, 2.22-, and 11.66-fold relative activities when working at a pH as low as 3, at a NaCl concentration as high as 500 mM, and at a trypsin concentration as high as 10 mg/mL. We find that SPC encapsulation accelerates the cascade reaction efficiency of HRP and glucose oxidase. Owing to SPCs enhancing the catalytic activity of the loaded enzymes, we established an amplified enzyme-linked immunosorbent assay (ELISA) for the detection of

Identifiers

PMID37033870
PMCPMC10077544
OpenAlexW4360608498

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.