ArticleBMC plant biology2023
The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L.
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- Establishment of Transient Expression and Hairy Root Induction Systems inPlants (Basel, Switzerland) · 2026Article
- Safflower injection against obesity-induced mice podocyte injury by improving insulin resistance through increasing renal INSR and eNOS expression.Renal failure · 2025Article
- An Efficient Agrobacterium-Mediated Transient Transformation System Using In Vitro Embryo-Derived Seedlings for Gene Function Elucidation inPlants (Basel, Switzerland) · 2025Article
- Establishment ofPlants (Basel, Switzerland) · 2025Article
- Melatonin Enhances Panax vietnamensis Resistance to Leaf Blight Pathogen Neofusicoccum ribis via the PvWRKY40-PvCOMT2 Module-Driven Lignin Biosynthesis.Molecular plant pathology · 2025Article
- Advancements in delivery strategies and non-tissue culture regeneration systems for plant genetic transformation.Advanced biotechnology · 2024Review
- The effects of different hormone combinations on the growth ofFrontiers in plant science · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSafflower (Carthamus tinctorius L.) is an important economic crop and a traditional medicinal material rich in flavonoids, which can alleviate cardiovascular and cerebrovascular pathologies. Thus, many candidate genes involved in safflower flavonoid biosynthesis have been cloned. However, owing to the lack of a homologous gene expression system, research on gene function is limited to model plants. Therefore, a gene function identification protocol for safflower must be established.
resultsIn the present study, using safflower callus as the experimental material, Agrobacterium and biolistic transient expression systems were established. In the Agrobacterium transient expression system, the highest transformation rate was obtained at the original Agrobacterium concentration of OD
conclusionUsing safflower callus as the experimental material, highly efficient Agrobacterium and biolistic transient expression systems were successfully established, and the utility of both systems for investigating gene function was demonstrated. The proposed safflower callus transient expression systems will be useful for further functional analyses of flavonoid biosynthetic genes in safflower.
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