ArticleScientific reports2023
First continuous marine sponge cell line established.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 21 citations in OpenAlex.
- Development of long-term oyster explant cultures enable sustained tissue-specific activity and reveal novel cellular behaviours.Biochemistry and biophysics reports · 2026Article
- Hypoxia-induced immortalization ofiScience · 2026Article
- Coating materials enhance urochordate primary cell culture adherence.In vitro cellular & developmental biology. Animal · 2026Article
- The Chemistry and Pharmacology of the Alkaloid Barettin and Its Analogues from the Marine SpongeMarine drugs · 2026Review
- MALDI-FISH for Colocalization of Brominated Metabolites andJournal of natural products · 2026Article
- Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025Review
- Challenges in cellular agriculture: lessons from Pacific white shrimp, Litopenaeus vannamei.In vitro cellular & developmental biology. Animal · 2025Article
- Review
- I-SMEL a Big Catch!ACS central science · 2023Article
- Whole genome sequence of the deep-sea sponge Geodia barretti (Metazoa, Porifera, Demospongiae).G3 (Bethesda, Md.) · 2023Article
- Improved Media Formulations for Primary Cell Cultures Derived from a Colonial Urochordate.Cells · 2023Article
- From friends to foes: fungi could be emerging marine sponge pathogens under global change scenarios.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The potential of sponge-derived chemicals for pharmaceutical applications remains largely unexploited due to limited available biomass. Although many have attempted to culture marine sponge cells in vitro to create a scalable production platform for such biopharmaceuticals, these efforts have been mostly unsuccessful. We recently showed that Geodia barretti sponge cells could divide rapidly in M1 medium. In this study we established the first continuous marine sponge cell line, originating from G. barretti. G. barretti cells cultured in OpM1 medium, a modification of M1, grew more rapidly and to a higher density than in M1. Cells in OpM1 reached 1.74 population doublings after 30 min, more than twofold higher than the already rapid growth rate of 0.74 population doublings in 30 min in M1. The maximum number of population doublings increased from 5 doublings in M1 to at least 98 doublings in OpM1. Subcultured cells could be cryopreserved and used to inoculate new cultures. With these results, we have overcome a major obstacle that has blocked the path to producing biopharmaceuticals with sponge cells at industrial scale for decades.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.