ArticleNature communications2023
Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 73 citations in OpenAlex.
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- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
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- Visualizing Dynamic Processes in Energy Materials by Interferometric Scattering Microscopy.ACS energy letters · 2026Review
- Interferometric Image Scanning Microscopy for label-free imaging at 120 nm lateral resolution inside live cells.Light, science & applications · 2026Article
- Temporal variance mapping with machine learning for label-free 3D chromatin imaging using optical interferometric microscopy.Biomedical optics express · 2026Article
- Meta-amplified dark-field interferometric scattering microscopy.Nature communications · 2026Article
- An integrated platform for liquid handling and cell imaging in life science applications.Nature communications · 2025Article
- Bidirectional quantitative scattering microscopy.Nature communications · 2025Article
- Leveraging Partial Coherence to Enhance Nanoparticle Detection Sensitivity and Throughput in Interferometric Scattering Microscopy.ACS photonics · 2025Article
- Multiparametric Characterization of Individual Suspended Nanoparticles Using Confocal Fluorescence and Interferometric Scattering Microscopy with Microfluidic Confinement.Nano letters · 2025Article
- Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics.iScience · 2025Article
- From Biophysics to Biomedical Physics.ACS bio & med chem Au · 2025Review
- Nanophotonic sensing and label-free imaging of extracellular vesicles.Light, science & applications · 2025Review
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- Real-time label-free imaging of living crystallization-driven self-assembly.Nature communications · 2025Article
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- Label-Free Anti-Brownian Trapping of Single Nanoparticles in Solution.The journal of physical chemistry. C, Nanomaterials and interfaces · 2024Article
- Surpassing the Diffraction Limit in Label-Free Optical Microscopy.ACS photonics · 2024Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bright-field light microscopy and related phase-sensitive techniques play an important role in life sciences because they provide facile and label-free insights into biological specimens. However, lack of three-dimensional imaging and low sensitivity to nanoscopic features hamper their application in many high-end quantitative studies. Here, we demonstrate that interferometric scattering (iSCAT) microscopy operated in the confocal mode provides unique label-free solutions for live-cell studies. We reveal the nanometric topography of the nuclear envelope, quantify the dynamics of the endoplasmic reticulum, detect single microtubules, and map nanoscopic diffusion of clathrin-coated pits undergoing endocytosis. Furthermore, we introduce the combination of confocal and wide-field iSCAT modalities for simultaneous imaging of cellular structures and high-speed tracking of nanoscopic entities such as single SARS-CoV-2 virions. We benchmark our findings against simultaneously acquired fluorescence images. Confocal iSCAT can be readily implemented as an additional contrast mechanism in existing laser scanning microscopes. The method is ideally suited for live studies on primary cells that face labeling challenges and for very long measurements beyond photobleaching times.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.