ReviewACS synthetic biology2023
Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.
Review in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed.
- The physicochemical design, structure and permeability of the JCVI-syn3A minimal cell membrane.Nature communications · 2026Article
- Automated synthetic cell-based screening for designed proteins with emergent functions.Nature communications · 2026Article
- Nanofluidics for Pioneering Synthetic Biology of Bottom-Up Cell-Free Molecular Systems.ACS synthetic biology · 2026Review
- Breaking the membrane heredity paradox through de novo protocell formation.Nature communications · 2026Article
- Systematic investigation of double emulsion dewetting dynamics for the robust production of giant unilamellar vesicles.Lab on a chip · 2026Article
- Cell encapsulated biomaterials for translational medicine.Bioactive materials · 2026Review
- Design biology and mind-body health.Journal, genetic engineering & biotechnology · 2025Review
- Orchestrating Self-Replication in Artificial Cells with Digital Microfluidics.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- ATP synthesis driven by atmospheric hydrogen concentrations.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Meeting Proceedings from 4th Minimal Cell Workshop: Exploring JCVI Minimal Cell Fundamental Insights and Integrative Applications.ACS synthetic biology · 2025Article
- Balancing Permeability and Stability: A Study of Hybrid Membranes for Synthetic Cells Using Lipids and PBd-Biomacromolecules · 2025Article
- Light-Triggered Protease-Mediated Release of Actin-Bound Cargo from Synthetic Cells.Advanced biology · 2025Article
- Synthetic syntrophy for adenine nucleotide cross-feeding between metabolically active nanoreactors.Nature nanotechnology · 2025Article
- Membrane-embedded CdaA is required for efficient synthesis of second messenger cyclic di-AMP.Communications biology · 2024Article
- The Potential of Artificial Cells Functioning underACS synthetic biology · 2024Article
- Light-triggered protease-mediated release of actin-bound cargo from synthetic cells.bioRxiv : the preprint server for biology · 2024Article
- Chemiosmotic nutrient transport in synthetic cells powered by electrogenic antiport coupled to decarboxylation.Nature communications · 2024Article
- Nucleated synthetic cells with genetically driven intercompartment communication.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Synthetic Vesicles for Sustainable Energy Recycling and Delivery of Building Blocks for Lipid BiosynthesisACS synthetic biology · 2024Article
- Leveraging Active Learning to Establish Efficient In Vitro Transcription and Translation from Bacterial Chromosomal DNA.ACS omega · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Life-like systems need to maintain a basal metabolism, which includes importing a variety of building blocks required for macromolecule synthesis, exporting dead-end products, and recycling cofactors and metabolic intermediates, while maintaining steady internal physical and chemical conditions (physicochemical homeostasis). A compartment, such as a unilamellar vesicle, functionalized with membrane-embedded transport proteins and metabolic enzymes encapsulated in the lumen meets these requirements. Here, we identify four modules designed for a minimal metabolism in a synthetic cell with a lipid bilayer boundary: energy provision and conversion, physicochemical homeostasis, metabolite transport, and membrane expansion. We review design strategies that can be used to fulfill these functions with a focus on the lipid and membrane protein composition of a cell. We compare our bottom-up design with the equivalent essential modules of JCVI-syn3a, a top-down genome-minimized living cell with a size comparable to that of large unilamellar vesicles. Finally, we discuss the bottlenecks related to the insertion of a complex mixture of membrane proteins into lipid bilayers and provide a semiquantitative estimate of the relative surface area and lipid-to-protein mass ratios (i.e., the minimal number of membrane proteins) that are required for the construction of a synthetic cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.