ArticleJournal of veterinary internal medicine
Effect of sample volume and time on rumen juice analysis in cattle.
Article in Journal of veterinary internal medicine. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Effect of chemically protected sodium butyrate on growth performance, serum indicators, rumen fermentation parameters, and microbiota of Gangba sheep.Animal bioscience · 2026Article
- Effects of triticale silage substitution for corn silage on growth performance, serum biomarkers, rumen fermentation, and rumen bacterial community of Pingliang Red Cattle.BMC microbiology · 2026Article
- Effects of grazing and housing system on growth performance, serum indices, rumen fermentation parameters, and microbial community in Gangba sheep.BMC microbiology · 2026Article
- Impact of ginger powder (Zingiber officinale) supplementation on the performance, biochemical parameters, antioxidant status, and rumen fermentation in Ossimi rams.Veterinary world · 2024Article
- Effect of sample volume and time on rumen juice analysis in cattle.Journal of veterinary internal medicineArticle
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Authors and funding
3 authors.
Funding
Abstract
backgroundRumen juice analysis (RJA) involves analysis of a 10mL sample within minutes after sampling. However, it can be challenging to collect 10 mL of rumen juice (RJ) from some ruminants, and clinical circumstances can delay RJA.
objectivesQuantify the effect of sample volume (2, 5, 10, 50, and 100 mL), and time-to-analysis (0, 30, and 60 minutes) on RJA. ANIMALS: Cannulated cow.
methodsObservational experimental study. Two liters of RJ were collected at 26 separate times. The samples were subdivided into 2 duplicates of each sample volume at each sampling time; and analyzed at 0, 30, and 60 minutes after collection. Rumen juice analysis included pH measurement, methylene blue reduction time (MBRT), and protozoal motility.
resultsThe pH of 2 and 5 mL samples was significantly (P = .01) higher than the pH of 50 and 100 mL samples at all time points. The MBRT was significantly lower (faster bacterial reduction) for 100 mL samples compared to all other samples at 0 minute and to 2, 5, and 50 mL samples at 30 min. The pH and MBRT at 60 minutes were significantly higher than at 0 minute for all volumes (P < .05 and P < .01, respectively). For large protozoa, small sample volumes (2 and 5 mL) had significantly lower protozoal motility (scores of 5 and 4.5, respectively) compared to 100 mL samples at 60 minutes (score of 4; P < .05). CONCLUSIONS AND CLINICAL IMPORTANCE: Interpretation of RJA could be affected by small sample volumes and delays to analysis. Sample volumes of ≥10 mL analyzed within 30 minutes after collection are recommended.
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