Evidence map›Paper›PMID 37026075›Full record

ArticleOpen veterinary journal2023

Identification of reference genes for expression studies in the liver and spleen of laying hens housed in cage and cage-free systems.

María P Herrera-Sánchez, Kelly J Lozano-Villegas, Iang S Rondón-Barragán, Roy Rodríguez-Hernández

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Open veterinary journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

María P Herrera-SánchezPoultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué, Colombia.
Kelly J Lozano-VillegasPoultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué, Colombia.
Iang S Rondón-BarragánPoultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué, Colombia.
Roy Rodríguez-HernándezPoultry Research Group, Laboratory of Immunology and Molecular Biology, Faculty of Veterinary Medicine and Zootechnics, University of Tolima, Ibagué, Colombia.
University of Tolima · CO

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: The liver and spleen play a pivotal role in metabolism and immune response. During stress, neuroendocrine response induces changes in gene expression, and its assessment demands the validation of the stability of the reference genes to perform relative gene expression experiments. Aim: The objective of this study was to determine the expression stability of four reference genes ( Methods: Liver and spleen from Hy-Line Brown hens housed in CC and CF egg production systems were used. mRNA transcript levels were determined by quantitative polymerase chain reaction (qPCR), and the gene expression stability was evaluated using geNorm, BestKeeper, and NormFinder algorithms. Results: The most stable gene from liver tissue was Conclusion: The

Indexed as

ChickensSpleenAnimalsFemaleLiverLaying hensLivermRNAReference genesSpleen

Identifiers

PMID37026075
PMCPMC10072828
OpenAlexW4327519943

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.