Evidence map›Paper›PMID 37025420›Full record

ArticleEpigenetics insights2023

Next Generation Sequencing for miRNA Detection on the Exhaled Breath Condensate: A Pilot Study.

Roberto Cherchi, Roberto Cusano, Sandro Orrù, Paolo A Ferrari, Matteo Massidda, Giorgio Fotia, Sara De Matteis, Pierluigi Cocco

Abstract read
In one paragraph

Article in Epigenetics insights, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Roberto CherchiOperative Unit of Thoracic Surgery, Hospital G. Brotzu, Cagliari, Italy.
Roberto CusanoCRS4-NGS Core, POLARIS Research Park, Pula-Cagliari, Italy.
Sandro OrrùOperative Unit of Medical Genetics, Health Agency of Sardinia, Hospital Binaghi, Cagliari, Italy.ORCID https://orcid.org/0000-0001-5085-7975
Paolo A FerrariOperative Unit of Thoracic Surgery, Hospital G. Brotzu, Cagliari, Italy.
Matteo MassiddaCRS4-NGS Core, POLARIS Research Park, Pula-Cagliari, Italy.
Giorgio FotiaCRS4-NGS Core, POLARIS Research Park, Pula-Cagliari, Italy.
Sara De MatteisDepartment of Medical Sciences and Public Health, University of Cagliari, Monserrato-Cagliari, Italy.
Pierluigi CoccoCentre for Occupational and Environmental Health, Division of Population Health, Health Services Research & Primary Care, University of Manchester, Manchester, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Exhaled breath condensate (EBC) sampling has been suggested as a less-invasive and cost-effective method to detect biological macromolecules, including miRNA. To explore the feasibility of its use as a biomarker of early effects of asbestos exposure, we conducted a preliminary test on male volunteers by comparing the miRNA profile in the EBC and the plasma using 2 different sequencing platforms. Methods: Six male volunteers, all retired and unexposed to dust or fumes, participated in the test. RNA was extracted from 200 μL EBC samples and same-size plasma samples. Sample aliquots were processed in 2 laboratories using 2 different sequencing platforms: a MiSeq Illumina Results: The HiSeq3000 Conclusion: Our preliminary study confirms that, when using the HiSeq

Indexed as

biomarkersexhaled breath condensatemiRNAnext generation sequencing

Identifiers

PMID37025420
PMCPMC10070752

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.