ArticleNature plants2023
An efficient CRISPR-Cas12a promoter editing system for crop improvement.
Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed.
- Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants.Journal of integrative plant biology · 2026Article
- Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026Review
- Optimized tRNA processing and TREX2-SpCas9 fusion enable high-efficiency multiplex genome editing in plants.Plant communications · 2026Article
- Single-Cell CRISPR: An Efficient Strategy for Decoding Plant Cis-Regulatory Complexity.Plant biotechnology journal · 2026Review
- Tuning Rice Gene Expression via In Situ Promoter Truncations Using a Prime Editing Library.Plant biotechnology journal · 2026Article
- Review
- Cis-regulatory editing of SD1 promoter enhances TCP19-mediated repression to optimize plant height in Kam sweet rice.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Article
- Mapping cis-regulatory mutations at scale in sorghum enables modulation of gene expression.Nature biotechnology · 2026Article
- From Natural Discovery to AI-Guided Design: A Curated Collection of Compact Enhancers for Crop Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Targeted Gene Modification ofInternational journal of molecular sciences · 2026Article
- Modulating the strigolactone pathway to optimize tomato shoot branching for vertical farming.Journal of integrative plant biology · 2026Article
- CRISPR/Cas9-mediated uORF engineering enhances tanshinone biosynthesis inHorticulture research · 2026Article
- Biotechnological and genetic innovations to enhance sorghum adaptation under climate change.Frontiers in plant science · 2026Review
- Engineering non-coding DNA Elements in Rice: an Elegant Approach To fine-tune Agronomical Advantageous Traits.Rice (New York, N.Y.) · 2025Review
- Fine tuning wheat heading time through genome editing of transcription factor binding sites in Ppd-1 gene promoter.Scientific reports · 2025Article
- The Potential of NGTs to Overcome Constraints in Plant Breeding and Their Regulatory Implications.International journal of molecular sciences · 2025Review
- Populus PtrbHLH011 Is a Transcriptional Co-Regulator Involved in the Activation of Cell Wall Biosynthesis by Iron Deprivation.Plant biotechnology journal · 2025Article
- Increasing Cas12a- and Cas9-mediated deletion sizes with exonucleases.Plant physiology · 2025Article
- Rational design of promoter editing confers multipathogen resistance in rice.Genome biology · 2025Article
- Protocol for permanent gene repression by CRISPR-adenine base editing of promoter CCAAT motifs.STAR protocols · 2025Article
26 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on byA ton of Plants.2023
Authors and funding
18 authors.
Funding
Abstract
Promoter editing represents an innovative approach to introduce quantitative trait variation (QTV) in crops. However, an efficient promoter editing system for QTV needs to be established. Here we develop a CRISPR-Cas12a promoter editing (CAPE) system that combines a promoter key-region estimating model and an efficient CRISPR-Cas12a-based multiplexed or singular editing system. CAPE is benchmarked in rice to produce QTV continuums for grain starch content and size by targeting OsGBSS1 and OsGS3, respectively. We then apply CAPE for promoter editing of OsD18, a gene encoding GA3ox in the gibberellin biosynthesis pathway. The resulting lines carry a QTV continuum of semidwarfism without significantly compromising grain measures. Field trials demonstrated that the OsD18 promoter editing lines have the same yield performance and antilodging phenotype as the Green Revolution OsSD1 mutants in different genetic backgrounds. Hence, promoter editing of OsD18 generates a quantitative Green Revolution trait. Together, we demonstrate a CAPE-based promoter editing and tuning pipeline for efficient production of useful QTV continuum in crops.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.