Evidence map›Paper›PMID 37022346›Full record

ArticleArchives of gynecology and obstetrics2023

Extending the culture of cleavage-stage embryos to the blastocyst stage after warming increases the chance of live birth: does it have a regenerative effect?

Göğşen Önalan, Mehmet Tunç, Aytaç Tohma, Emre Günakan, Tahir Eryılmaz, Hulusi B Zeyneloğlu

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Article in Archives of gynecology and obstetrics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Göğşen ÖnalanDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey.ORCID 0000-0003-3446-3751
Mehmet TunçDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey. mhmttunc@gmail.com.ORCID 0000-0002-8646-0619
Aytaç TohmaDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey.ORCID 0000-0001-9418-4733
Emre GünakanDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey.ORCID 0000-0001-8854-8190
Tahir EryılmazDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey.ORCID 0000-0001-7029-6159
Hulusi B ZeyneloğluDepartment of Obstetrics and Gynecology, Başkent University School of Medicine, Şehit Temel Kuğuoğlu Cd. No: 34 Bahçelievler, Ankara, Turkey.ORCID 0000-0002-0289-2642

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeWe aimed to evaluate the effect of extending the culture of cleavage-stage embryos to the blastocyst stage in vitrified-warmed cycles on pregnancy outcomes.

methodsThis is a retrospectively designed pilot study of a single center. All patients who applied for freeze-all cycle procedures during in vitro fertilization treatment were included in the study. Patients were classified into three subgroups. The embryos obtained were frozen at the cleavage or blastocyst stage. After a warming process, the cleavage-stage embryos were divided into two subgroups: the first group of embryos was transferred (vitrification day 3-embryo transfer (ET) day 3 (D3T3)) on the warming day; for the second group, the embryo culture was extended to the blastocyst stage (vitrification day 3-ET day 5 (after the extension of the embryo culture to the blastocyst stage), (D3T5)). Frozen blastocyst-stage embryos were transferred after warming (vitrification day 5-ET day 5 (D5T5)). Hormone replacement treatment was the only endometrial preparation regimen given during the embryo transfer cycle. The main outcome of the study was live birth rates. The clinical pregnancy rate and positive pregnancy test rate were determined as the secondary outcomes of the study.

resultsThe study included a total of 194 patients. The positive pregnancy test rates (PPR) and clinical pregnancy rates (CPR) of the D3T3, D3T5, and D5T5 groups were 14.0% and 59.2%; 43.8% and 9.3%; and 56.3% and 39.6%, respectively (p < 0.001 and p < 0.001). The live birth rates (LBR) of patients in the D3T3, D3T5, and D5T5 groups were 7.0%, 44.7%, and 27.1%, respectively (p < 0.001). In subgroup analysis of patients with a poor number of 2PN embryos (defined as having <  = 4 2PN embryos), the D3T5 group had significantly higher PPR (10.7%, 60.6%, 42.4%; p < 0.001), CPR (7.1%, 57.6%, 39.4%; p < 0.001), and LBR (3.6%, 39.4%, 21.2%; p: 0.001).

conclusionExtending the culture after warming to the blastocyst stage may be a better alternative than a cleavage-stage embryo transfer.

Indexed as

BlastocystCryopreservationLive BirthFemaleHumansPilot ProjectsPregnancyPregnancy RateRetrospective StudiesVitrificationBlastocystCleavageFreeze allThawVitrification

Identifiers

PMID37022346

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.