Evidence map›Paper›PMID 37022163›Full record

ArticleJournal of virology2023

Reconstitution and Mutagenesis of Avian Infectious Laryngotracheitis Virus from Cosmid and Yeast Centromeric Plasmid Clones.

Stephen Spatz, Maricarmen García, Walter Fuchs, Carlos Loncoman, Jeremy Volkening, Teresa Ross, Sylva Riblet, Taejoong Kim, Nathan Likens, Thomas Mettenleiter

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of virology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 4 citations in OpenAlex.

  1. Synthetic nucleic acids in a post-agent biosecurity Era.Frontiers in bioengineering and biotechnology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 5 institutions in 3 countries.

Stephen SpatzUS National Poultry Research Center, Athens, Georgia, USA.ORCID 0000-0002-1545-5855
Maricarmen GarcíaPoultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Walter FuchsInstitute of Molecular Virology and Cell Biology, Friedrich Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.ORCID 0000-0002-7670-451X
Carlos LoncomanAsia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Veterinary and Agricultural Sciences, University of Melbourne, Parkville, Victoria, Australia.
Jeremy VolkeningBASE2BIO, Oshkosh, Wisconsin, USA.
Teresa RossUS National Poultry Research Center, Athens, Georgia, USA.
Sylva RibletPoultry Diagnostic and Research Center, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.
Taejoong KimUS National Poultry Research Center, Athens, Georgia, USA.
Nathan LikensUS National Poultry Research Center, Athens, Georgia, USA.
Thomas MettenleiterInstitute of Molecular Virology and Cell Biology, Friedrich Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald-Insel Riems, Germany.
U.S. National Poultry Research Center · USFriedrich-Loeffler-Institut · DEUniversity of Georgia · USOshkosh (United States) · USThe University of Melbourne · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The genomes of numerous herpesviruses have been cloned as infectious bacterial artificial chromosomes. However, attempts to clone the complete genome of infectious laryngotracheitis virus (ILTV), formally known as Gallid alphaherpesvirus-1, have been met with limited success. In this study, we report the development of a cosmid/yeast centromeric plasmid (YCp) genetic system to reconstitute ILTV. Overlapping cosmid clones were generated that encompassed 90% of the 151-Kb ILTV genome. Viable virus was produced by cotransfecting leghorn male hepatoma (LMH) cells with these cosmids and a YCp recombinant containing the missing genomic sequences - spanning the TR

Indexed as

CosmidsHerpesvirus 1, GallidMutagenesisPlasmidsAnimalsCell LineChickensGenome, ViralHerpesviridae InfectionsMalePoultry DiseasesSaccharomyces cerevisiaeViral ProteinsViral Proteinsalphaherpesviruscosmid librariesGallid alphaherpesvirus-1infectious laryngotracheitis virusorigin of DNA replicationpackaging sitesyeast centromeric plasmids

Identifiers

PMID37022163
PMCPMC10134816
OpenAlexW4362657014

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.