ArticleMolecular plant2023
Calcium-dependent activation of CPK12 facilitates its cytoplasm-to-nucleus translocation to potentiate plant hypoxia sensing by phosphorylating ERF-VII transcription factors.
Article in Molecular plant, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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42 citing papers in PubMed, 78 citations in OpenAlex.
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- Genome-wide identification and evolutionary analysis of the ERF-VII gene family in the tea plant (Camellia sinensis) and functional characterization of CsRAP2.2 in response to cold stress.Plant cell reports · 2026Article
- Flooding stress resilience and crop improvement.Plant cell reports · 2026Review
- Duet between sugars and hormones: The molecular dialogue fine-tuning hypoxia acclimation in plants.Journal of integrative plant biology · 2026Review
- Chemical priming of hypoxia responses via PLANT CYSTEINE OXIDASE inhibition improves flooding tolerance.Plant physiology · 2026Article
- JrCDPK13L-mediated phosphorylation of JrERF113L promotes walnut resistance to Colletotrichum gloeosporioides.Plant physiology · 2026Article
- Natural variation inScience advances · 2026Article
- The CPK3-mediated cytosolic-nuclear translocation of bHLH107 recruits HY5 to regulate the CuJournal of advanced research · 2026Article
- Transcriptome analysis reveals the mechanism and key role of CdPER47 in bermudagrass (Cynodon dactylon) adaptation to submergence and post-submergence stresses.BMC plant biology · 2026Article
- The SnRK1-RAP2.4h-PIP2 module contributes to the trade-off between growth and hypoxia tolerance in plants.Nature communications · 2026Article
- Prolyl hydroxylase-dependent proteolysis enables the orthogonal hypoxia responses in plants.Nature communications · 2026Article
- MITOGEN-ACTIVATED PROTEIN KINASE9 fine-tunes hypoxia signaling by phosphorylating ERF-VII transcription factors in Arabidopsis.The New phytologist · 2026Article
- Transcriptome-Based Dissection of the Molecular Mechanisms Underlying Flooding Stress Responses of Eastern Cottonwood in the Floodplains of the Middle and Lower Reaches of the Yangtze River.Plants (Basel, Switzerland) · 2026Article
- Dynamic Coordination: How ERF Transcription Factors Coordinate Plant Development and Adaptive Stress Responses.Biomolecules · 2026Review
- A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A phosphorylation-dependent ubiquitination switch orchestrates nuclear immune reprogramming upon chitin perception.Nature communications · 2026Article
- Natural variation in Phosphatidylinositol 4-Kinase OsPI4Kγ7 and its interaction with OsLIC balance rice yield and latitudinal adaptation.Nature communications · 2026Article
- Integrated morpho-physiological and transcriptomic dissection of waterlogging tolerance inFrontiers in plant science · 2026Article
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- G-Box Factors 14-3-3 Proteins Negatively RegulatePlants (Basel, Switzerland) · 2025Article
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11 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Calcium-dependent protein kinases (CDPKs/CPKs) are key regulators of plant stress signaling that translate calcium signals into cellular responses by phosphorylating diverse substrate proteins. However, the molecular mechanism by which plant cells relay calcium signals in response to hypoxia remains elusive. Here, we show that one member of the CDPK family in Arabidopsis thaliana, CPK12, is rapidly activated during hypoxia through calcium-dependent phosphorylation of its Ser-186 residue. Phosphorylated CPK12 shuttles from the cytoplasm to the nucleus, where it interacts with and phosphorylates the group VII ethylene-responsive transcription factors (ERF-VII) that are core regulators of plant hypoxia sensing, to enhance their stabilities. Consistently, CPK12 knockdown lines show attenuated tolerance of hypoxia, whereas transgenic plants overexpressing CPK12 display improved hypoxia tolerance. Nonethelss, loss of function of five ERF-VII proteins in an erf-vii pentuple mutant could partially suppress the enhanced hypoxia-tolerance phenotype of CPK12-overexpressing lines. Moreover, we also discovered that phosphatidic acid and 14-3-3κ protein serve as positive and negative modulators of the CPK12 cytoplasm-to-nucleus translocation, respectively. Taken together, these findings uncover a CPK12-ERF-VII regulatory module that is key to transducing calcium signals from the cytoplasm into the nucleus to potentiate hypoxia sensing in plants.
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