ArticleNature2023
Cryo-EM structure of the transposon-associated TnpB enzyme.
Abstract read
In one paragraph
Article in Nature, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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74citing papers in PubMed
–field-weighted citation impact
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Who cites it
74 citing papers in PubMed.
- One Plasmid Is All You Need: Genome Editing in Escherichia coli Using Endogenous TnpB and Endogenous Recombination System.Biotechnology and bioengineering · 2026Article
- De novo-engineered guide RNA-directed transposition with TnpB-family proteins.Molecular cell · 2026Article
- Structural and genetic dissection of RNA-guided gene repression by TnpB-derived transcription factors.bioRxiv : the preprint server for biology · 2026Article
- Structure-guided discovery and engineering of miniature CRISPR-Cas12m for epigenome editing.Nature structural & molecular biology · 2026Article
- Review
- Large-scale mutational analysis uncovers molecular mechanisms governing dual RNA functions in transposons.Nucleic acids research · 2026Article
- Dual-mode application of transcriptional regulation and DNA editing using the ultra-compact TnpB system.Nucleic acids research · 2026Article
- Stepwise DNA-unwinding gates TnpB genome-editing activity.Molecular cell · 2026Article
- The Single-Stranded DNA Platform: A Potential Broad-Spectrum Vector for Editing Genes in Prokaryotes: ssDNA May Break Through the Difficulties of Genetic Manipulation on Non-Model Bacteria.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Structure and evolution-guided design of minimal RNA-guided nucleases.Science (New York, N.Y.) · 2026Article
- Directed evolution of compact RNA-guided nucleases for enhanced activity in mammalian cells.Genome biology · 2026Article
- Engineering high-efficiency mutants of the transposase ISDra2 TnpB via protein-generative models and energy-optimized design.aBIOTECH · 2026Article
- Miniature and versatile genome regulation TnpB-ωRNA toolkits facilitate cancer immunotherapy.Nature communications · 2026Article
- Engineered TnpB genome editors for plants and human cells identified by ribonucleoprotein mutational scanning.Nature biotechnology · 2026Article
- Structural visualization of the molecular evolution of CRISPR-Cas9.Nature structural & molecular biology · 2026Article
- Engineering hypercompact IscB nucleases for efficient and versatile genome editing in rice.Genome biology · 2026Article
- Comprehensive assessment of activity, specificity, and safety of hypercompact TnpB systems for gene editing.Genome biology · 2026Article
- Engineering the MmeFz2-ωRNA system for efficient genome editing through an integrated computational-experimental framework.Nature communications · 2026Article
- Stepwise DNA unwinding gates TnpB genome-editing activity.bioRxiv : the preprint server for biology · 2026Article
- Advances and challenges in single-cell RNA sequencing data analysis: a comprehensive review.Briefings in bioinformatics · 2026Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
22 authors.
Ryoya Nakagawa *Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Hisato Hirano *Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Satoshi N OmuraDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Suchita NetyBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-4201-1061
Soumya KannanBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-7332-9662
Han Altae-TranBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Xiao YaoDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Yuriko SakaguchiDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Takayuki OhiraDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0002-1487-1312
Wen Y WuLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.
Hiroshi NakayamaBiomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Saitama, Japan.
Yutaro ShutoDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Tatsuki TanakaDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Fumiya K SanoDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Tsukasa KusakizakoDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.ORCID http://orcid.org/0000-0002-6186-6647
Yoshiaki KiseDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Yuzuru ItohDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
Naoshi DohmaeBiomolecular Characterization Unit, RIKEN Center for Sustainable Resource Science, Saitama, Japan.ORCID http://orcid.org/0000-0002-5242-9410
John van der OostLaboratory of Microbiology, Wageningen University and Research, Wageningen, The Netherlands.ORCID http://orcid.org/0000-0001-5024-1871
Tsutomu SuzukiDepartment of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, Tokyo, Japan.
Feng ZhangBroad Institute of MIT and Harvard, Cambridge, MA, USA.ORCID http://orcid.org/0000-0003-0178-7995
Osamu NurekiDepartment of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan. nureki@bs.s.u-tokyo.ac.jp.ORCID http://orcid.org/0000-0003-1813-7008
Funding
Medical Scientist Training ProgramT32GM007753 · NIGMS · HARVARD UNIVERSITY (MEDICAL SCHOOL) · PI WALENSKY, LOREN DAVID · 1985 to 2021
$50.0MMedical Scientist Training ProgramT32GM144273 · NIGMS · HARVARD MEDICAL SCHOOL · PI David Shumway Jones, Jacqueline A. Lees · 2022 to 2026
$14.7MProgrammable RNA-targeting toolsR01HG009761 · NHGRI · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2017 to 2024
$7.5MNHGRI NIH HHS R01 HG009761NIGMS NIH HHS T32 GM007753NIGMS NIH HHS T32 GM144273
Abstract
The class 2 type V CRISPR effector Cas12 is thought to have evolved from the IS200/IS605 superfamily of transposon-associated TnpB proteins
Indexed as
Bacterial ProteinsCryoelectron MicroscopyDeinococcusDNA Transposable ElementsEndodeoxyribonucleasesCRISPR-Associated ProteinsCRISPR-Cas SystemsDNARNA, Guide, CRISPR-Cas SystemsSubstrate SpecificityBacterial ProteinsCRISPR-Associated ProteinsDNADNA Transposable ElementsEndodeoxyribonucleasesRNA, Guide, CRISPR-Cas Systems
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Read underepoch 390
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.