ArticleScientific reports2023
Identification of two insecticide resistance markers in Ethiopian Anopheles stephensi mosquitoes using a multiplex amplicon sequencing assay.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 20 citations in OpenAlex.
- Insecticide resistance in mosquitoes: Current status, mechanism, monitoring and innovative strategies for sustainable vector control.Molecular biology reports · 2026Review
- Susceptibility of the invasive malaria vector Anopheles stephensi in Ethiopia to novel chemical insecticides and insect growth regulator.Scientific reports · 2025Article
- A Short-Read Amplicon Sequencing Protocol and Bioinformatic Pipeline for Ecological Surveillance of Dipteran Disease Vectors.Molecular ecology resources · 2025Article
- Profiling insecticide resistance phenotypes and genotypes in Aedes aegypti populations across four regions in Puerto Rico.Scientific reports · 2025Article
- Genome wide population genetics and molecular surveillance of insecticide resistance in Anopheles stephensi mosquitoes from Awash Sebat Kilo in Ethiopia.Scientific reports · 2025Article
- The origin, invasion history and resistance architecture ofbioRxiv : the preprint server for biology · 2025Article
- Omics Approaches in Understanding Insecticide Resistance in Mosquito Vectors.International journal of molecular sciences · 2025Review
- MhGeneS: An Analytical Pipeline to Allow for Robust Microhaplotype Genotyping.Molecular ecology resources · 2025Article
- Article
- Insecticide susceptibility status of Anopheles stephensi against novel insecticides in Eastern Ethiopia.Research square · 2024Article
- Genomic surveillance of Anopheles mosquitoes on the Bijagós Archipelago using custom targeted amplicon sequencing identifies mutations associated with insecticide resistance.Parasites & vectors · 2024Article
- First detection ofbioRxiv : the preprint server for biology · 2023Article
- Rapid profiling of Plasmodium parasites from genome sequences to assist malaria control.Genome medicine · 2023Article
- Detection of insecticide resistance markers in Anopheles funestus from the Democratic Republic of the Congo using a targeted amplicon sequencing panel.Scientific reports · 2023Article
- Towards environmental detection, quantification, and molecular characterization of Anopheles stephensi and Aedes aegypti from experimental larval breeding sites.Scientific reports · 2023Article
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Authors and funding
11 authors at 4 institutions in 4 countries.
Funding
Abstract
Since its first detection in 2012 in Djibouti, Anopheles stephensi has invaded and established in the Horn of Africa, and more recently Nigeria. The expansion of this vector poses a significant threat to malaria control and elimination efforts. Integrated vector management is the primary strategy used to interrupt disease transmission; however, growing insecticide resistance is threatening to reverse gains in global malaria control. We present a next-generation amplicon-sequencing approach, for high-throughput monitoring of insecticide resistance genes (ace1, GSTe2, vgsc and rdl), species identification and characterization of genetic diversity (its2 and cox1) in An. stephensi. Ninety-five An. stephensi mosquitoes, collected in Ethiopia, were screened, identifying 104 SNPs, including the knock-down mutation L958F (L1014F in Musca domestica), and for the first time in this vector species, the A296S substitution (A301S in Drosophila melanogaster) in the rdl locus. Two other amino acid substitutions (ace1-N177D, GSTe2-V189L) were also identified but have not been previously implicated in insecticide resistance. Genetic diversity in the mitochondrial cox1 gene revealed shared haplotypes between Ethiopian An. stephensi with samples from Pakistan, Sudan, and Djibouti. Overall, we present a reliable, cost-effective strategy using amplicon-sequencing to monitor known insecticide resistance mutations, with the potential to identify new genetic variants, to assist in the high-throughput surveillance of insecticide resistance in An. stephensi populations.
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