ArticleNature communications2023
Ultrafast light targeting for high-throughput precise control of neuronal networks.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed.
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- A hybrid micro-ECoG for functionally targeted multi-site and multi-scale investigation.Cell reports methods · 2026Article
- Engineering Neuronal Network Connectivity Through Precise and Scalable Electrical Modulation.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Chromatix: a differentiable, GPU-accelerated wave-optics library.Nature methods · 2026Article
- The Importance of Synchrony in the Neural Control of Movement.bioRxiv : the preprint server for biology · 2026Article
- Theoretical analysis of low power synergistic sono-optogenetic control of calcium-dependent synaptic plasticity.Scientific reports · 2026Article
- An Open-Source Pipeline for Calcium Imaging and All-Optical Physiology in Human Stem Cell-Derived Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A hybrid micro-ECoG for functionally targeted multi-site and multi-scale investigation.bioRxiv : the preprint server for biology · 2026Article
- Chromatix: a differentiable, GPU-accelerated wave-optics library.bioRxiv : the preprint server for biology · 2026Article
- A Closed-Loop-Capable Neural Interface Platform for Deep Brain Modulation via Integrated Non-Viral Gene Delivery, NIR Optogenetics, and Electrophysiological Recording.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Traceless Regulation of Genetic Circuitry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Technical development of two-photon optogenetic stimulation and its potential application to brain-machine interfaces.Neurophotonics · 2026Review
- High-throughput synaptic connectivity mapping using in vivo two-photon holographic optogenetics and compressive sensing.Nature neuroscience · 2025Article
- Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits.ACS photonics · 2025Review
- Three-dimensional scanless patterned illumination using time-multiplexed multiline temporal focusing for multicell manipulation with single-cell resolution.Journal of biomedical optics · 2025Article
- Theoretical analysis of low power optogenetic control of synaptic plasticity with subcellular expression of CapChR2 at postsynaptic spine.Scientific reports · 2025Article
- Reversible light-responsive protein hydrogel for on-demand cell encapsulation and release.Acta biomaterialia · 2025Article
- Two-photon all-optical neurophysiology for the dissection of larval zebrafish brain functional and effective connectivity.Communications biology · 2024Article
- Recent advances in light patterned optogenetic photostimulation in freely moving mice.Neurophotonics · 2024Article
- Demixing fluorescence time traces transmitted by multimode fibers.Nature communications · 2024Article
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
Two-photon, single-cell resolution optogenetics based on holographic light-targeting approaches enables the generation of precise spatiotemporal neuronal activity patterns and thus a broad range of experimental applications, such as high throughput connectivity mapping and probing neural codes for perception. Yet, current holographic approaches limit the resolution for tuning the relative spiking time of distinct cells to a few milliseconds, and the achievable number of targets to 100-200, depending on the working depth. To overcome these limitations and expand the capabilities of single-cell optogenetics, we introduce an ultra-fast sequential light targeting (FLiT) optical configuration based on the rapid switching of a temporally focused beam between holograms at kHz rates. We used FLiT to demonstrate two illumination protocols, termed hybrid- and cyclic-illumination, and achieve sub-millisecond control of sequential neuronal activation and high throughput multicell illumination in vitro (mouse organotypic and acute brain slices) and in vivo (zebrafish larvae and mice), while minimizing light-induced thermal rise. These approaches will be important for experiments that require rapid and precise cell stimulation with defined spatio-temporal activity patterns and optical control of large neuronal ensembles.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.