Evidence map›Paper›PMID 37017784›Full record

ArticleArchives of microbiology2023

Morphological and molecular comparison as a useful tool for identification of the three centric marine diatoms (Bacillariophyceae: Chaetoceros).

Maliwan Kutako, Rachanimuk Hiransuchalert, Sarayut Watchasit, Mookthida Kaewduang, Orawan Hanchana, Pakawan Setthamongkol, Parinya Chindudsadeegul, Vichaya Gunbua, Somtawin Jaritkhuan

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Archives of microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Maliwan KutakoMarine Biotechnology Research Unit, Faculty of Marine Technology, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand.
Rachanimuk HiransuchalertMarine Biotechnology Research Unit, Faculty of Marine Technology, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand. rachanimuk@buu.ac.th.ORCID http://orcid.org/0000-0002-7929-2100
Sarayut WatchasitNuclear Magnetic Resonance Spectroscopic Laboratory, Science Innovation Facility, Faculty of Science, Burapha University, 169 Long-Hard Bangsaen Road, Saensook, Muang, Chon Buri, 20131, Thailand.
Mookthida KaewduangMarine Biotechnology Research Unit, Faculty of Marine Technology, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand.
Orawan HanchanaMarine Biotechnology Research Unit, Faculty of Marine Technology, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand.
Pakawan SetthamongkolMarine Biotechnology Research Unit, Faculty of Marine Technology, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand.
Parinya ChindudsadeegulFaculty of Gems, Burapha University, Chanthaburi Campus, 57 Moo 1, Kamong, Thamai, Chanthaburi, 22170, Thailand.
Vichaya GunbuaDepartment of Aquatic Science, Faculty of Science, Burapha University, 169 Long-Hard Bangsaen Road, Saensook, Muang, Chon Buri, 20131, Thailand.
Somtawin JaritkhuanDepartment of Aquatic Science, Faculty of Science, Burapha University, 169 Long-Hard Bangsaen Road, Saensook, Muang, Chon Buri, 20131, Thailand.
Burapha University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this study was to identify morphological and molecular comparison of three marine Chaetoceros species using microscopic observations, sequence analysis of 18S rDNA, random amplified polymorphic DNA (RAPD-PCR) barcoding and nuclear magnetic resonance (NMR) spectroscopy. Chaetoceros were obtained from three different algae laboratories: Center of Excellence for Marine Biotechnology (CEMB), Chanthaburi Coastal Fisheries Research and Development (CHAN) and Institute of Marine Science, Burapha University (BIM). Genomic DNA for the RAPD-PCR analysis was extracted using the phenol-chloroform method, followed by 18S rDNA amplification. The blast results of 18S rDNA sequence confirmed the significantly matched to C. gracilis for Chaetoceros BIM and CHAN and C. muelleri for Chaetoceros CEMB(e-value = 0.0, identity = 99%). The RAPD-PCR results revealed differences in the three Chaetoceros isolates with polymorphisms between 30.43% and 60.00%, and Chaetoceros CEMB showed high polymorphic bands. Scanning electron microscopy revealed that Chaetoceros CEMB were larger and had larger setae compared to the other isolates (P < 0.05). The results of the NMR characterization of metabolites were consistent with the results of the sequence and morphological analyses. The concentrations of several metabolites, including chlorophyll c

Indexed as

DiatomsChlorophyll ADNA, RibosomalHumansMicroscopy, Electron, ScanningRandom Amplified Polymorphic DNA TechniqueChlorophyll ADNA, Ribosomal18S rDNA sequenceChaetocerosMorphologyNMRRAPD-PCR

Identifiers

PMID37017784
PMCPMC10075179
OpenAlexW4362601775

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.