Evidence map›Paper›PMID 37017732›Full record

ArticleApplied microbiology and biotechnology2023

Beauveria bassiana exhibits strong virulence against Dendroctonus ponderosae in greenhouse and field experiments.

Kleinberg X Fernandez, Stanley Pokorny, Guncha Ishangulyeva, Aziz Ullah, Silvia I Todorova, Nadir Erbilgin, Allan L Carroll, John C Vederas

Abstract read
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In one paragraph

Article in Applied microbiology and biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
2.2field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 9 citations in OpenAlex.

  1. Detoxification of conifer antimicrobial defenses promotes entomopathogenic fungus infection of bark beetles.Proceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Kleinberg X FernandezDepartment of Chemistry, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, T6G 2G2, Canada.ORCID http://orcid.org/0000-0003-1836-0162
Stanley PokornyDepartment of Forest and Conservation Sciences, University of British Columbia, 3219-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.
Guncha IshangulyevaDepartment of Renewable Resources, University of Alberta, 11223 Saskatchewan Drive, Edmonton, AB, T6G 2E3, Canada.ORCID http://orcid.org/0000-0003-1898-7125
Aziz UllahDepartment of Renewable Resources, University of Alberta, 11223 Saskatchewan Drive, Edmonton, AB, T6G 2E3, Canada.ORCID http://orcid.org/0000-0001-6155-8874
Silvia I TodorovaAnatis Bioprotection, 278 Rang Saint-André, Saint-Jacques-le-Mineur, QC, J0J 1Z0, Canada.
Nadir ErbilginDepartment of Renewable Resources, University of Alberta, 11223 Saskatchewan Drive, Edmonton, AB, T6G 2E3, Canada.ORCID http://orcid.org/0000-0001-9912-8095
Allan L CarrollDepartment of Forest and Conservation Sciences, University of British Columbia, 3219-2424 Main Mall, Vancouver, BC, V6T 1Z4, Canada.ORCID http://orcid.org/0000-0003-1820-0759
John C VederasDepartment of Chemistry, University of Alberta, 11227 Saskatchewan Drive, Edmonton, AB, T6G 2G2, Canada. john.vederas@ualberta.ca.ORCID http://orcid.org/0000-0002-2996-0326
University of Alberta · CAUniversity of British Columbia · CAHôpital Saint-Jacques · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The mountain pine beetle (MPB) has infested over 16 million hectares of pine forests in western Canada, killing over 50% of mature lodgepole pine, Pinus contorta, in British Columbia alone. There are few tools available to manage irruptive bark beetle populations and to mitigate tree mortality. Beauveria bassiana is an entomopathogenic fungus that causes mortality to several bark beetle species. However, the potential for B. bassiana as a biocontrol agent against pine beetle populations is unknown. We selected three strains of B. bassiana from several culture collections and evaluated their conidial stability under cold storage, in planta (greenhouse, and pine bolts) and in natura (forest stand, pine bolts, and live pines) conditions. The stability assays showed that all fungal strains maintained a minimum effective conidial yield through the assay durations (3-12 weeks). In addition, we adapted a biphasic liquid-solid fermentation approach for the large-scale production of conidial biomass, yielding up to a 100-fold increase in production. In greenhouse virulence assays, the mean lethal time of MPBs was reduced to 3-4 days upon treatment with B. bassiana, where high B. bassiana-associated mycosis was also observed. Furthermore, the application of B. bassiana formulation substantially affected the gallery network of MPBs in bolts in the field, resulting in shorter larval galleries and significantly reduced offspring production. Indeed, high titer treatments reduced the mean larvae per gallery to virtually zero. Together these results demonstrate that B. bassiana may be a viable biocontrol tool to reduce mountain pine beetle populations in pine forests in western Canada. KEY POINTS: • Three B. bassiana strains identified to be stable at various test conditions. • Large-scale conidial biomass production using liquid-solid biphasic fermentation. • Reproductive success of D. ponderosae significantly reduced by B. bassiana formulation.

Indexed as

BeauveriaColeopteraPinusAnimalsForestsLarvaSpores, FungalVirulenceBark beetlesEntomopathogensForest entomologyMicrobial biocontrol agentsMountain pine beetle

Identifiers

PMID37017732
OpenAlexW4362602450

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.