Evidence map›Paper›PMID 37017722›Full record

ArticleAnalytical and bioanalytical chemistry2023

In situ droplet-based on-tissue chemical derivatization for lipid isomer characterization using LESA.

Dallas P Freitas, Xi Chen, Erin A Hirtzel, Madison E Edwards, Joohan Kim, Hongying Wang, Yuxiang Sun, Klaudia I Kocurek, David Russell, Xin Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical and bioanalytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Spatial mapping of phosphatidylcholineInternational journal of mass spectrometry · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Dallas P FreitasDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Xi ChenDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Erin A HirtzelDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Madison E EdwardsDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Joohan KimDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Hongying WangDepartment of Nutrition, Texas A&M University, Carter-Mattil Hall, 373 Olven Blvd, College Station, TX, 77843, USA.
Yuxiang SunDepartment of Nutrition, Texas A&M University, Carter-Mattil Hall, 373 Olven Blvd, College Station, TX, 77843, USA.
Klaudia I KocurekDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
David RussellDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA.
Xin YanDepartment of Chemistry, Texas A&M University, 580 Ross St, College Station, TX, 77843, USA. xyan@tamu.edu.ORCID http://orcid.org/0000-0002-8292-130X

Funding

Waters Select Series Cyclic IMS for P41 Native MS Resource Application to Alzheimer's DiseaseP41GM128577 · NIGMS · OHIO STATE UNIVERSITY · PI WYSOCKI, VICKI H. · 2018 to 2022
$7.6M
Panoptic electrochemical probe for next-generation mass spectrometry based-lipidomicsR35GM143047 · NIGMS · TEXAS A&M UNIVERSITY · PI Xin Yan · 2021 to 2026
$2.6M
Nutrient-sensing GHS-R in macrophage reprogramming and inflamm-agingR01AG064869 · NIA · TEXAS A&M AGRILIFE RESEARCH · PI SUN, YUXIANG · 2019 to 2023
$2.1M
NIA NIH HHS R01 AG064869NIGMS NIH HHS P41 GM128577NIGMS NIH HHS R35 GM143047NIGMS NIH HHS R35GM143047
6 · The paper itself

Abstract

In this work, we present an in situ droplet-based derivatization method for fast tissue lipid profiling at multiple isomer levels. On-tissue derivatization for isomer characterization was achieved in a droplet delivered by the TriVersa NanoMate LESA pipette. The derivatized lipids were then extracted and analyzed by the automated chip-based liquid extraction surface analysis (LESA) mass spectrometry (MS) followed by tandem MS to produce diagnostic fragment ions to reveal the lipid isomer structures. Three reactions, i.e., mCPBA epoxidation, photocycloaddition catalyzed by the photocatalyst Ir[dF(CF

Indexed as

LipidsTandem Mass SpectrometryAnimalsIsomerismMiceLipidsDropletLipid isomerLiquid extraction surface analysisMass spectrometryOn-tissue chemical derivatization

Identifiers

PMID37017722
PMCPMC10392465

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.