ArticleInvestigative ophthalmology & visual science2023
On the Mechanical Roles of Glycosaminoglycans in the Tensile Properties of Porcine Corneal Stroma.
Article in Investigative ophthalmology & visual science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
- High-yield cell-derived extracellular matrix bioink via macromolecular crowding for versatile 3D bioprinting.Materials today. Bio · 2026Article
- Microstructure and In-Plane Mechanical Property Comparison of Human and Porcine Cornea.Journal of biomechanical engineering · 2025Article
- Insights into chondrocyte populations in cartilaginous tissues at the single-cell level.Nature reviews. Rheumatology · 2025Review
- Effect of Hydration on Viscoelastic Tensile Properties of Sclera.Vision (Basel, Switzerland) · 2025Article
- Corneal Biomechanical Changes Induced by Chronic Elevated Intraocular Pressure: Implications for Glaucoma Pathogenesis and Management.Journal of ophthalmology · 2025Review
- A pilot study for decellularizing porcine cornea for future use in corneal regeneration.PloS one · 2025Article
- The relationship between keratan sulfate glycosaminoglycan density and mechanical stiffening of CXL treatment.Experimental eye research · 2023Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
Purpose: The cornea is primarily composed of collagen fibrils that are embedded in a ground substance rich in proteoglycans and other glycoproteins. It is known that glycosaminoglycan (GAG) side chains of proteoglycans form anti-parallel duplexes between collagen fibrils. The present work was done in order to investigate the mechanical function of GAGs in defining the tensile properties of porcine corneal stroma. Methods: Porcine corneal stromal strips dissected from the nasal-temporal direction were divided into control, buffer-treated, and enzyme-treated groups. The samples in the control group were used immediately after dissection. However, the buffer-treated and enzyme-treated samples were, respectively, incubated for 18 hours at 37°C in a buffer solution made up of 100-mM sodium acetate at pH 6.0 or in an enzyme solution containing keratanase II. The Blyscan assay was used to quantify the total GAG content and assess GAG depletion in the samples treated with the enzyme and buffer solutions. Uniaxial tensile tests were also performed to determine the effect of GAG removal on mechanical properties of the cornea. Results: The GAG content in enzyme-treated samples was significantly lower than that of the normal and buffer-treated specimens (P < 0.05). Moreover, GAG-depleted strips showed significantly softer mechanical responses in comparison with the control and buffer samples (P < 0.05). Conclusions: Removing GAGs from the corneal extracellular matrix led to significant tensile property reduction; supporting the hypothesis that there exists a strong correlation between the GAG content and mechanical properties of the corneal stroma.
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Registered trials
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