ArticleEuropean biophysics journal : EBJ2023
A new UltraScan module for the characterization and quantification of analytical buoyant density equilibrium experiments to determine AAV capsid loading.
Article in European biophysics journal : EBJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Predictive model for evolving density and viscosity gradients in band-forming ultracentrifugation.European biophysics journal : EBJ · 2025Article
- Methods for the Design and Analysis of Analytical Ultracentrifugation Experiments.Current protocols · 2024Article
- Characterization and quantification of adeno-associated virus capsid-loading states by multi-wavelength analytical ultracentrifugation with UltraScan.Nanomedicine (London, England) · 2023Article
- AAV analysis by sedimentation velocity analytical ultracentrifugation: beyond empty and full capsids.European biophysics journal : EBJ · 2023Article
- Proceedings of the 25th Analytical Ultracentrifugation Workshops and Symposium.European biophysics journal : EBJ · 2023Article
- Optimizing high-throughput viral vector characterization with density gradient equilibrium analytical ultracentrifugation.European biophysics journal : EBJ · 2023Article
- Proceedings of the 25th Analytical Ultracentrifugation Workshops and Symposium.European biophysics journal : EBJ · 2023Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
A method for characterizing and quantifying peaks formed in an analytical buoyant density equilibrium (ABDE) experiment is presented. An algorithm is derived to calculate the concentration of the density forming gradient material at every point in the cell, provided the rotor speed, temperature, meniscus position, bottom of the cell position, and the loading concentration, molar mass, and partial specific volume of the density gradient-forming material are known. In addition, a new peak fitting algorithm has been developed which allows the user to automatically quantify the peaks formed in terms of density, apparent partial specific volume, and relative abundance. The method is suitable for both ionic and non-ionic density forming materials and can be used with data generated from the UV optical system as well as the AVIV fluorescence optical system. These methods have been programmed in a new UltraScan-III module (us_abde). Examples are shown that demonstrate the application of the new module to adeno-associated viral vector preparations and proteins.
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Registered trials
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