ArticleMolecular medicine (Cambridge, Mass.)2023
Cryopreserved placental biopsies maintain mitochondrial activity for high-resolution respirometry.
Article in Molecular medicine (Cambridge, Mass.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Assessing Mitochondrial Respiratory Complex-Associated Function From Previously Frozen Mouse Placental Tissue.Bio-protocol · 2026Article
- Unlocking the potential of mitochondrial transplantation: overcoming challenges and paving the way for routine therapeutic application.Cytotechnology · 2025Review
- Chronic stress elicits sex-specific mitochondrial respiratory functional changes in the rat heart.Physiological reports · 2025Article
- Culture of cryopreserved first trimester placental tissues to study syncytial renewal.Scientific reports · 2025Article
- Impact of cryopreservation on immune cell metabolism as measured by SCENITH.Oxford open immunology · 2025Article
- Placental Bioenergetics and Antioxidant Homeostasis in Maternal Obesity and Gestational Diabetes.Antioxidants (Basel, Switzerland) · 2024Article
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Authors and funding
6 authors.
Funding
Abstract
backgroundHigh-resolution respirometry (HRR) of human biopsies can provide useful metabolic, diagnostic, and mechanistic insights for clinical research and comparative medical studies. Fresh tissues analysis offers the potential best condition, the drawback being the need to use them shortly after dissection for mitochondrial respiratory experiments. The development of effective long-term storage protocols for biopsies that allow the assessment of key Electron Transport System (ETS) parameters at later stages is thus a major need.
methodsWe optimised a cryopreservation protocol that preserves mitochondrial membranes intactness, otherwise affected by direct tissue freezing. The protocol is based on a gradual freezing step from on-ice to liquid nitrogen and - 80 °C storage using a specific DMSO-based buffer.
resultsPlacenta is a suitable tissue to design and test the effectiveness of long-term storage protocols being metabolically active foetal tissue with mitochondrial dysfunctions contributing to placental disease and gestational disorders. Here we designed and tested the effectiveness of the cryopreservation protocol using human placenta biopsies; we measured the ETS activity by HRR of placenta specimens comparing fresh, cryopreserved, and snap frozen conditions.
conclusionsBy this protocol, Oxygen Consumption Rate (OCR) measurements of fresh and cryopreserved placental specimens are comparable whereas snap frozen procedure impairs mitochondrial activity.
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