Evidence map›Paper›PMID 37012505›Full record

ArticleMedical molecular morphology2023

FGF4 and FGF9 have synergistic effects on odontoblast differentiation.

Tatsuki Hoshino, Shoko Onodera, Motoyoshi Kimura, Makoto Suematsu, Tatsuya Ichinohe, Toshifumi Azuma

Abstract read
PubMed Publisher
In one paragraph

Article in Medical molecular morphology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Tatsuki HoshinoDepartment of Dental Anesthesiology, Tokyo Dental College, Misaki-cho, Chiyoda-ku, Tokyo, Japan.
Shoko OnoderaDepartment of Biochemistry, Tokyo Dental College, 2-9-18, Kanda-Misakichou, Chiyoda-ku, Tokyo, 101-0061, Japan.
Motoyoshi KimuraDepartment of Pediatric Dentistry, Tokyo Dental College, Misaki-cho, Chiyoda-ku, Tokyo, Japan.
Makoto SuematsuDepartment of Dental Biochemistry, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo, Japan.
Tatsuya IchinoheDepartment of Dental Anesthesiology, Tokyo Dental College, Misaki-cho, Chiyoda-ku, Tokyo, Japan.
Toshifumi AzumaDepartment of Biochemistry, Tokyo Dental College, 2-9-18, Kanda-Misakichou, Chiyoda-ku, Tokyo, 101-0061, Japan. tazuma@tdc.ac.jp.ORCID http://orcid.org/0000-0002-0746-8138
Tokyo Dental College · JPKeio University · JP

Funding

Exploratory Research for Advanced Technology Suematsu Gas BiologyJapan Society for the Promotion of Science 18H03007Japan Society for the Promotion of Science 19K19074Japan Society for the Promotion of Science 21H03146
6 · The paper itself

Abstract

The purpose of this study was to investigate whether fibroblast growth factor 4 (FGF4) and FGF9 are active in dentin differentiation. Dentin matrix protein 1 (Dmp1) -2A-Cre transgenic mice, which express the Cre-recombinase in Dmp1-expressing cells, were crossed with CAG-tdTomato mice as reporter mouse. The cell proliferation and tdTomato expressions were observed. The mesenchymal cell separated from neonatal molar tooth germ were cultured with or without FGF4, FGF9, and with or without their inhibitors ferulic acid and infigratinib (BGJ398) for 21 days. Their phenotypes were evaluated by cell count, flow cytometry, and real-time PCR. Immunohistochemistry for FGFR1, 2, and 3 expression and the expression of DMP1 were performed. FGF4 treatment of mesenchymal cells obtained promoted the expression of all odontoblast markers. FGF9 failed to enhance dentin sialophosphoprotein (Dspp) expression levels. Runt-related transcription factor 2 (Runx2) was upregulated until day 14 but was downregulated on day 21. Compared to Dmp1-negative cells, Dmp1-positive cells expressed higher levels of all odontoblast markers, except for Runx2. Simultaneous treatment with FGF4 and FGF9 had a synergistic effect on odontoblast differentiation, suggesting that they may play a role in odontoblast maturation.

Indexed as

Core Binding Factor Alpha 1 SubunitFibroblast Growth Factor 4Fibroblast Growth Factor 9OdontoblastsAnimalsCell DifferentiationMiceMice, TransgenicCore Binding Factor Alpha 1 SubunitFgf4 protein, mouseFgf9 protein, mouseFibroblast Growth Factor 4Fibroblast Growth Factor 9Dmp1Enamel knotFGF4FGF9Mesenchymal cellsOdontoblast

Identifiers

PMID37012505
OpenAlexW4362521425

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.