Evidence map›Paper›PMID 37010767›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Monitoring the Activation of Selective Autophagy via N-Terminal Arginylation.

Chang Hoon Ji, Su Bin Kim, Min Ju Lee, Yong Tae Kwon

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Chang Hoon JiCellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Jongno-gu, Seoul, Republic of Korea.
Su Bin KimCellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Jongno-gu, Seoul, Republic of Korea.
Min Ju LeeCellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Jongno-gu, Seoul, Republic of Korea.
Yong Tae KwonCellular Degradation Biology Center and Department of Biomedical Sciences, College of Medicine, Seoul National University, Jongno-gu, Seoul, Republic of Korea. yok5@snu.ac.kr.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In addition to generating N-degron-carrying substrates destined for proteolysis, N-terminal arginylation can globally upregulate selective macroautophagy via activation of the autophagic N-recognin and archetypal autophagy cargo receptor p62/SQSTM1/sequestosome-1. To evaluate the macroautophagic turnover of cellular substrates, including protein aggregates (aggrephagy) and subcellular organelles (organellophagy) mediated by N-terminal arginylation in vivo, we report here a protocol for assaying the activation of the autophagic Arg/N-degron pathway and degradation of cellular cargoes via N-terminal arginylation. These methods, reagents, and conditions are applicable across a wide spectrum of different cell lines, primary cultures, and/or animal tissues, thereby providing a general means for identification and validation of putative cellular cargoes degraded by Nt-arginylation-activated selective autophagy.

Indexed as

AutophagyMacroautophagyAnimalsEndoplasmic ReticulumHeLa CellsHumansProteolysisSequestosome-1 ProteinSequestosome-1 ProteinATE1Autophagic fluxDetergent-insoluble/soluble fractionationIn vitro p62/SQSTM1 self-oligomerizationNt-arginylationPunctate formation/co-localization

Identifiers

PMID37010767

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.