ArticleAnalytical chemistry2023
Laser-Based Mid-Infrared Spectroscopy for Monitoring Temperature-Induced Denaturation of Bovine Serum Albumin and De-/Stabilization Effects of Sugars.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 21 citations in OpenAlex.
- Too close for comfort: Self-crowding transforms protein structure and stability beyond volume exclusion.Protein science : a publication of the Protein Society · 2026Article
- Non-nutritive sweeteners in food-drug interactions: An overview of current evidence.Molecular pharmacology · 2025Review
- Integrated Optics Waveguides and Mesoporous Oxides for the Monitoring of Volatile Organic Compound Traces in the Mid-Infrared.Applied spectroscopy · 2025Article
- Mannose-Glycated Metal-Phenolic Microcapsules Orchestrate Phenotype Switch of Macrophages for Boosting Tumor Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Innovative Infrared Spectroscopic Technologies for the Prediction of Deoxynivalenol in Wheat.ACS food science & technology · 2025Article
- Microbubble-Templated Immunoactive Metal-Phenolic Capsules for Drug Delivery and Enhanced Cancer Immunotherapy.Research (Washington, D.C.) · 2025Article
- External Cavity Quantum Cascade Laser Vibrational Circular Dichroism Spectroscopy for Fast and Sensitive Analysis of Proteins at Low Concentrations.Analytical chemistry · 2024Article
- Ratiometric luminescent sensor based on BSA-coated gold/silver nanoclusters for the selective determination and spatiotemporal imaging of gallic acid in plants.Mikrochimica acta · 2023Article
- Single-detector double-beam modulation for high-sensitivity infrared spectroscopy.Scientific reports · 2023Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stability of high-concentration protein formulations is considered a major challenge in current biopharmaceutical development. In this work, we introduce laser-based mid-infrared (IR) spectroscopy as a versatile technique to study the effect of protein concentration and presence of sugars on the thermal denaturation of the model protein bovine serum albumin (BSA). Many analytical techniques struggle to characterize the complex structural transition that occurs during protein denaturation. To this end, a commercially available laser-based mid-IR spectrometer equipped with a customized flow cell was employed to record IR spectra of BSA in the temperature range of 25-85 °C. The temperature perturbation induces a conformational change from a native α-helical to an intermolecular β-sheet secondary structure in BSA. Systematic investigation of the concentration dependence of the α-β transition temperature between 30 and 90 mg mL
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