Evidence map›Paper›PMID 37009624›Full record

ArticleMicrobial cell (Graz, Austria)2023

A modular cloning (MoClo) toolkit for reliable intracellular protein targeting in the yeast

Pavel Simakin, Christian Koch, Johannes M Herrmann

Open access · goldAbstract read
In one paragraph

Article in Microbial cell (Graz, Austria), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Pavel SimakinCell Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany.
Christian KochCell Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany.
Johannes M HerrmannCell Biology, University of Kaiserslautern, 67663 Kaiserslautern, Germany.
University of Kaiserslautern · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Modular Cloning (MoClo) allows the combinatorial assembly of plasmids from standardized genetic parts without the need of error-prone PCR reactions. It is a very powerful strategy which enables highly flexible expression patterns without the need of repetitive cloning procedures. In this study, we describe an advanced MoClo toolkit that is designed for the baker's yeast

Indexed as

expression plasmidsmitochondrial importmodular cloningpromoter strengthprotein targetingsplit GFP

Identifiers

PMID37009624
PMCPMC10054711
OpenAlexW4353108918

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.