Evidence map›Paper›PMID 37007980›Full record

ArticleJournal of biological methods2023

Proximity Protein Labeling In

Jamie A Takashima, Helena A Woroniecka, Pascale G Charest

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biological methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Jamie A TakashimaDepartment of Chemistry and Biochemistry, University of Arizona, Tucson AZ, USA.
Helena A WoronieckaDepartment of Chemistry and Biochemistry, University of Arizona, Tucson AZ, USA.
Pascale G CharestDepartment of Chemistry and Biochemistry, University of Arizona, Tucson AZ, USA.
University of Arizona · USCalifornia Institute of Technology · US

Funding

CHEMISTRY-BIOLOGY INTERFACE TRAINING PROGRAMT32GM008804 · NIGMS · UNIVERSITY OF ARIZONA · PI MONTFORT, WILLIAM R. · 2003 to 2023
$3.9M
The role and regulation of mTORC2 in cell migrationR01GM131200 · NIGMS · UNIVERSITY OF ARIZONA · PI CHAREST, PASCALE G · 2019 to 2023
$1.6M
NIGMS NIH HHS R01 GM131200NIGMS NIH HHS T32 GM008804
6 · The paper itself

Abstract

To fully understand any cellular process, we not only need to identify the proteins implicated, but also how the protein network is structurally and spatially organized and changes over time. However, the dynamic nature of many protein interactions involved in cellular signaling pathways continues to be the bottleneck in mapping and studying protein networks. Fortunately, a recently developed proximity labeling method using engineered ascorbic acid peroxidase 2 (APEX2) in mammalian cells allows the identification of weak and/or transient protein interactions with spatial and temporal resolution. Here, we describe a protocol for successfully using the APEX2-proximity labeling method in

Indexed as

APEXBiotinylationDictyostelium discoideumEngineered ascorbic peroxidaseProtein-protein interactionProximity labeling

Identifiers

PMID37007980
PMCPMC10062472
OpenAlexW4327619299

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.