ArticleBMC microbiology2023
Whole genome sequencing of a novel Bacillus thuringiensis isolated from Assam soil.
Article in BMC microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
13 citing papers in PubMed, 19 citations in OpenAlex.
- Genomic and Functional Characterization of the EndophyticMicroorganisms · 2026Article
- Complete genome sequence ofMicrobiology resource announcements · 2026Article
- Intestinal-Protective Lactiplantibacillus plantarum DPU-MWM14-103 Exhibits Genomic Adaptations and Multifaceted Probiotic Properties.Probiotics and antimicrobial proteins · 2026Article
- Decoding the genome of Bacillus thuringiensis subsp. israelensis VCRC B668 to understand its mosquitocidal potential for biopesticide development.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Decoupling sporulation from insecticidal activity inFrontiers in microbiology · 2026Article
- Genomic Insights IntoBioinformatics and biology insights · 2026Article
- Bacillus altitudinis: a sustainable mosquito controlling biopesticide isolated from the rhizospheric soil of Nypa fruticans in mangrove forest.BMC microbiology · 2025Article
- Comprehensive Genomic Insights and Insecticidal Evaluation of Bacillus thuringiensis Strain T419 against Lepidopteran Pests.Current microbiology · 2025Article
- Screening, Identification, and Whole-Genome Sequencing of Ferulic Acid Esterase-Producing Lactic Acid Bacteria from Sheep Rumen.Microorganisms · 2025Article
- Whole genome sequencing and evolutionary significance of a novel mosquitocidal bacterium, Bacillus thuringiensis serovar israelensis VCRC-B650 reported from Union Territory of Puducherry, India highly useful for mosquito control.Molecular genetics and genomics : MGG · 2025Article
- Genomic and Transcriptomic Profiling ofFoods (Basel, Switzerland) · 2025Article
- Bacillus thuringiensis Bacteremia in a 30-Year-Old Intravenous Drug User: A Report of a Rare Case.Cureus · 2024Article
- An overview of the production and use ofOpen life sciences · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundBacillus thuringiensis (Bt) is a gram-positive ubiquitous saprophytic bacterium that produces proteins (Crystal protein, Vegetative insecticidal protein, and Secreted insecticidal protein) toxic to insects during its growth cycle. In the present study, the whole genome of a locally isolated B. thuringiensis strain BA04 was sequenced to explore the genetic makeup and to identify the genes responsible to produce insecticidal proteins including the virulence factors. The strain was isolated from the soil sample of the Kaziranga National Park, Assam, North-Eastern part of India (Latitude: 26°34'39.11''N and Longitude: 93°10'16.04''E).
resultsThe whole genome sequencing (WGS) of the BA04 strain revealed that it has a circular genome of size 6,113,005 bp with four numbers of plasmids. A total of 6,111 genes including two novel crystal protein-encoding genes (MH753362.1 and MH753363.1) were identified. The BLASTn analysis of MH753362.1 showed 84% similarities (maximum identity) with Cry1Ia (KJ710646.1) gene, whereas MH753363.1 exhibited 66% identity with Insecticidal Crystal Protein (ICP)-6 gene (KM053257.1). At the protein level, MH753362.1 and MH753363.1 shared 79% identity with Cry1Ia (AIW52613.1) and 40% identity with Insecticidal Crystal Protein (ICP)-6 (AJW76687.1) respectively. Three-dimensional structures of these two novel protein sequences revealed that MH753362.1 have 48% structural similarity with Cry8ea1 protein, whereas MH753363.1 showed only 20% structural similarity with Cry4Aa protein. Apart from these insecticidal genes, the strain was also found to contain virulence and virulence-associated factors including the antibiotic resistance genes and Clustered regularly interspaced short palindromic repeat (CRISPR) sequences.
conclusionThis is the first report on the whole genome sequence of Bt strain BA04 isolated from Assam, a North-Eastern state of India. The WGS of strain BA04 unveils the presence of two novel types of insecticidal crystal protein-encoding genes which can be used for the development of insect-resistant transgenic crops. Additionally, the strain could be used for the formulations of effective biopesticides. The WGS provides the fastest and cheapest platform for a better understanding of the genetic makeup of a strain and helps to explore the role of virulence genes in pathogenicity against the insect host.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.