Evidence map›Paper›PMID 37002449›Full record

ArticleThe protein journal2023

Expression of Xanthorhodopsin in Escherichia coli.

Lada E Petrovskaya, Evgeniy P Lukashev, Ekaterina N Lyukmanova, Mikhail A Shulepko, Elena A Kryukova, Rustam H Ziganshin, Dmitriy A Dolgikh, Evgeniy G Maksimov, Andrei B Rubin, Mikhail P Kirpichnikov and 2 more

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Article in The protein journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Lada E PetrovskayaLaboratory of Protein Engineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya, 16/10, Moscow, 117997, Russia. lpetr65@yahoo.com.
Evgeniy P LukashevDepartment of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Ekaterina N LyukmanovaFaculty of Biology, Shenzhen MSU-BIT University, Shenzhen, 518172, China.
Mikhail A ShulepkoFaculty of Biology, Shenzhen MSU-BIT University, Shenzhen, 518172, China.
Elena A KryukovaGroup of Nanobioengineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, 117997, Russia.
Rustam H ZiganshinGroup of Mass Spectrometry, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Moscow, Russia.
Dmitriy A DolgikhLaboratory of Protein Engineering, Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, ul. Miklukho-Maklaya, 16/10, Moscow, 117997, Russia.
Evgeniy G MaksimovDepartment of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Andrei B RubinDepartment of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Mikhail P KirpichnikovDepartment of Biology, Lomonosov Moscow State University, Moscow, 119991, Russia.
Janos K LanyiDepartment of Physiology and Biophysics, D-320, Medical Sciences I, University of California, Irvine, CA, 92697-4560, USA.
Sergei P BalashovDepartment of Physiology and Biophysics, D-320, Medical Sciences I, University of California, Irvine, CA, 92697-4560, USA. balashov@uci.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Xanthorhodopsin (XR) from Salinibacter ruber is a light-driven proton pump containing retinal and a light-harvesting carotenoid antenna salinixanthin. Previous structure-functional studies of XR were conducted using a protein isolated from the native host only due to the absence of heterologous expression in Escherichia coli. In this paper, we describe cell-free synthesis and incorporation in lipid-protein nanodiscs of the recombinant XR that demonstrated its principal compatibility with E. coli biosynthetic machinery. To produce XR in E. coli, three C-terminal deletion variants of this protein were constructed. In contrast to the full-length XR, their expression resulted in efficient synthesis in E. coli cells. However, cells producing recombinant XR variants bound retinal only upon growth in minimal medium, not in the rich one. The XR3 variant with deletion of ten C-terminal amino acid residues was obtained and characterized. Its absorption spectrum and photocycle kinetics were close to those reported for XR isolated from S. ruber membranes and bleached from salinixanthin. We have also constructed the first mutants of XR, H62M and D96N, and examined their properties.

Indexed as

CarotenoidsEscherichia coliBacterial ProteinsGlycosidesBacterial ProteinsCarotenoidsGlycosidessalinixanthinCell-free synthesisE. coli expressionMutagenesisPhotocycleRetinal proteinXanthorhodopsin

Identifiers

PMID37002449

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.