Evidence map›Paper›PMID 37000316›Full record

ArticleApoptosis : an international journal on programmed cell death2023

MicroRNA-22-3p in human umbilical cord mesenchymal stem cell-secreted exosomes inhibits granulosa cell apoptosis by targeting KLF6 and ATF4-ATF3-CHOP pathway in POF mice.

Tian Gao, Ying Chen, Min Hu, Yi Cao, Ying Du

Abstract read
PubMed Publisher
In one paragraph

Article in Apoptosis : an international journal on programmed cell death, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
4.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
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  3. Primary Ovarian Insufficiency: Molecular Mechanisms and the Role of MicroRNAs.Reproductive sciences (Thousand Oaks, Calif.) · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Tian GaoDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. gaotiandoctor@hotmail.com.
Ying ChenReproductive Medicine Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China. chenying@cqmu.edu.cn.
Min HuReproductive Medicine Center, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Yi CaoDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
Ying DuDepartment of Gynecology and Obstetrics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.
The Affiliated Yongchuan Hospital of Chongqing Medical University · CNChongqing Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman umbilical cord mesenchymal stem cells (hUCMSCs)-derived exosomes carrying microRNAs (miRNAs) have promising therapeutic potential in various disorders, including premature ovarian failure (POF). Previous evidence has revealed the low plasma level of miR-22-3p in POF patients. Nevertheless, exosomal miR-22-3p specific functions underlying POF progression are unclarified.

methodsA cisplatin induced POF mouse model and in vitro murine ovarian granulosa cell (mOGC) model were established. Exosomes derived from miR-22-3p-overexpressed hUCMSCs (Exos-miR-22-3p) were isolated. CCK-8 assay and flow cytometry were utilized for measuring mOGC cell viability and apoptosis. RT-qPCR and western blotting were utilized for determining RNA and protein levels. The binding ability between exosomal miR-22-3p and Kruppel-like factor 6 (KLF6) was verified using luciferase reporter assay. Hematoxylin-eosin staining, ELISA, and TUNEL staining were performed for examining the alteration of ovarian function in POF mice.

resultsExos-miR-22-3p enhanced mOGC viability and attenuated mOGC apoptosis under cisplatin treatment. miR-22-3p targeted KLF6 in mOGCs. Overexpressing KLF6 reversed the above effects of Exos-miR-22-3p. Exos-miR-22-3p ameliorated cisplatin-triggered ovarian injury in POF mice. Exos-miR-22-3p repressed ATF4-ATF3-CHOP pathway in POF mice and cisplatin-treated mOGCs.

conclusionExosomal miR-22-3p from hUCMSCs alleviates OGC apoptosis and improves ovarian function in POF mouse models by targeting KLF6 and ATF4-ATF3-CHOP pathway.

Indexed as

ExosomesMesenchymal Stem CellsMicroRNAsPrimary Ovarian InsufficiencyActivating Transcription Factor 3Activating Transcription Factor 4AnimalsApoptosisCisplatinFemaleGranulosa CellsHumansKruppel-Like Factor 6MiceUmbilical CordActivating Transcription Factor 3Activating Transcription Factor 4ATF3 protein, humanATF4 protein, humanCisplatinKLF6 protein, humanKruppel-Like Factor 6MicroRNAsMIRN22 microRNA, humanGranulosa cellshUCMSCsKLF6miR-22-3pPremature ovarian failure

Identifiers

PMID37000316
OpenAlexW4362459621

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.