Evidence map›Paper›PMID 36997817›Full record

ArticleNature methods2023

High-resolution line-scan Brillouin microscopy for live imaging of mechanical properties during embryo development.

Carlo Bevilacqua, Juan Manuel Gomez, Ulla-Maj Fiuza, Chii Jou Chan, Ling Wang, Sebastian Hambura, Manuel Eguren, Jan Ellenberg, Alba Diz-Muñoz, Maria Leptin and 1 more

Abstract read
In one paragraph

Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed.

  1. Article
  2. Article
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  5. Marine Model Organisms for Mechanobiology Studies.Results and problems in cell differentiation · 2026
    Review
  6. Macrophages Mediate Mesoscale Brain Mechanical Homeostasis.Advanced materials (Deerfield Beach, Fla.) · 2026
    Article
  7. Article
  8. Review
  9. Untangling the fusion of spatial omics and mechanobiology.Progress in biomedical engineering (Bristol, England) · 2025
    Review
  10. Article
  11. Article
  12. A model for boundary-driven tissue morphogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  13. RAB5A Promotes Active Fluid Wetting by Reprogramming Breast Cancer Spheroid Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Review
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Carlo BevilacquaCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0001-9084-0641
Juan Manuel GomezDirector's Research Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Ulla-Maj FiuzaCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8691-0325
Chii Jou ChanCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Ling WangCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-7225-2878
Sebastian HamburaCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0002-8779-8515
Manuel EgurenCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Jan EllenbergCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0001-5909-701X
Alba Diz-MuñozCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany.ORCID http://orcid.org/0000-0001-6864-8901
Maria LeptinDirector's Research Unit, European Molecular Biology Laboratory, Heidelberg, Germany.
Robert PrevedelCell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany. prevedel@embl.de.ORCID http://orcid.org/0000-0003-3366-4703

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brillouin microscopy can assess mechanical properties of biological samples in a three-dimensional (3D), all-optical and hence non-contact fashion, but its weak signals often lead to long imaging times and require an illumination dosage harmful for living organisms. Here, we present a high-resolution line-scanning Brillouin microscope for multiplexed and hence fast 3D imaging of dynamic biological processes with low phototoxicity. The improved background suppression and resolution, in combination with fluorescence light-sheet imaging, enables the visualization of the mechanical properties of cells and tissues over space and time in living organism models such as fruit flies, ascidians and mouse embryos.

Indexed as

Embryonic DevelopmentMicroscopyAnimalsDrosophilaEmbryo, NonmammalianImaging, Three-DimensionalMice

Identifiers

PMID36997817
PMCPMC10172129

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.