ArticleNature biotechnology2024
Reflective multi-immersion microscope objectives inspired by the Schmidt telescope.
Article in Nature biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Glycolytic suppression of cell adhesion promotes thyroid morphogenesis in response to nutrition.EMBO reports · 2026Article
- High numerical aperture confocal volumetric mesoscope reveals mesoscale subcellular dynamics in vivo.Nature biotechnology · 2026Article
- Precise, predictable genome integrations by deep-learning-assisted design of microhomology-based templates.Nature biotechnology · 2026Article
- Refractive Index-Corrected Light-Sheet Microscopy for Macro-View Cardiovascular Imaging.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
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- Revolutionizing Tissue Clearing and 3-Dimensional Imaging: Transparent Embedding Solvent System for Uniform High-Resolution Imaging.BME frontiers · 2025Review
- SOLID: minimizing tissue distortion for brain-wide profiling of diverse architectures.Nature communications · 2024Article
- Ultra-long-working-distance multiphoton objective unlocks new possibilities for imaging.Nature methods · 2024Article
- Imagining the future of optical microscopy: everything, everywhere, all at once.Communications biology · 2023Review
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Authors and funding
14 authors.
Funding
Abstract
Imaging large, cleared samples requires microscope objectives that combine a large field of view (FOV) with a long working distance (WD) and a high numerical aperture (NA). Ideally, such objectives should be compatible with a wide range of immersion media, which is challenging to achieve with conventional lens-based objective designs. Here we introduce the multi-immersion 'Schmidt objective' consisting of a spherical mirror and an aspherical correction plate as a solution to this problem. We demonstrate that a multi-photon variant of the Schmidt objective is compatible with all homogeneous immersion media and achieves an NA of 1.08 at a refractive index of 1.56, 1.1-mm FOV and 11-mm WD. We highlight its versatility by imaging cleared samples in various media ranging from air and water to benzyl alcohol/benzyl benzoate, dibenzyl ether and ethyl cinnamate and by imaging of neuronal activity in larval zebrafish in vivo. In principle, the concept can be extended to any imaging modality, including wide-field, confocal and light-sheet microscopy.
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