Evidence map›Paper›PMID 36997631›Full record

ArticleScientific reports2023

Compact and automated eDNA sampler for in situ monitoring of marine environments.

Andre Hendricks, Connor M Mackie, Edward Luy, Colin Sonnichsen, James Smith, Iain Grundke, Mahtab Tavasoli, Arnold Furlong, Robert G Beiko, Julie LaRoche and 1 more

Abstract read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Review
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Field Trials of an Autonomous eDNA Sampler in Lotic Waters.Environmental science & technology · 2024
    Article
  15. Review
  16. Article
  17. Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Andre Hendricks *Department of Electrical and Computer Engineering, Dalhousie University, Halifax, NS, Canada.
Connor M Mackie *Dartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Edward LuyDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Colin SonnichsenDepartment of Electrical and Computer Engineering, Dalhousie University, Halifax, NS, Canada.
James SmithDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Iain GrundkeDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Mahtab TavasoliDepartment of Pharmacology, Dalhousie University, Halifax, NS, Canada.
Arnold FurlongDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Robert G BeikoDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Julie LaRocheDartmouth Ocean Technologies Inc, Dartmouth, NS, Canada.
Vincent SiebenDepartment of Electrical and Computer Engineering, Dalhousie University, Halifax, NS, Canada. sieben@dal.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Using environmental DNA (eDNA) to monitor biodiversity in aquatic environments is becoming an efficient and cost-effective alternative to other methods such as visual and acoustic identification. Until recently, eDNA sampling was accomplished primarily through manual sampling methods; however, with technological advances, automated samplers are being developed to make sampling easier and more accessible. This paper describes a new eDNA sampler capable of self-cleaning and multi-sample capture and preservation, all within a single unit capable of being deployed by a single person. The first in-field test of this sampler took place in the Bedford Basin, Nova Scotia, Canada alongside parallel samples taken using the typical Niskin bottle collection and post-collection filtration method. Both methods were able to capture the same aquatic microbial community and counts of representative DNA sequences were well correlated between methods with R[Formula: see text] values ranging from 0.71-0.93. The two collection methods returned the same top 10 families in near identical relative abundance, demonstrating that the sampler was able to capture the same community composition of common microbes as the Niskin. The presented eDNA sampler provides a robust alternative to manual sampling methods, is amenable to autonomous vehicle payload constraints, and will facilitate persistent monitoring of remote and inaccessible sites.

Indexed as

DNA, EnvironmentalMicrobiotaBiodiversityDNA Barcoding, TaxonomicEnvironmental MonitoringFiltrationHumansNova ScotiaDNA, Environmental

Identifiers

PMID36997631
PMCPMC10063616

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.