ArticleScientific reports2023
Alternative culture medium design for biomass production of autochthonous meat starter Latilactobacillus sakei sp. sakei ACU-2.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Lactococcus lactis I7 isolated from traditional Italian cheese making: a biotechnological integrated platform.BMC biotechnology · 2025Article
- Cost-effective medium optimization and functional enhancement ofVeterinary world · 2025Article
- Industrial production and functional profiling of probiotic Pediococcus acidilactici 72 N for potential use as a swine feed additive.Scientific reports · 2025Article
- Improving the survival under gastric conditions of a potential multistrain probiotic produced in co-culture.AMB Express · 2025Article
- Inhibition of Biogenic Amines in Fermented Tilapia Surimi by Collaborative Fermentation ofFoods (Basel, Switzerland) · 2024Article
- Design of an agro-industrial by-products-based media for the production of probiotic bacteria for fish nutrition.Scientific reports · 2024Article
- Article
- Production of a potential multistrain probiotic in co-culture conditions using agro-industrial by-products-based medium for fish nutrition.BMC biotechnology · 2023Article
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5 authors.
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Abstract
The autochthonous strain Latilactobacillus sakei sp. sakei ACU-2 was selected as a meat starter culture for dry sausage production. Transferring this strain from laboratory scale to industry requires an increase in biomass production, while lowering process costs. In this study, a combination of techniques was applied in order to optimize the culture medium composition to enhance biomass production of L. sakei ACU-2. One variable at a time experiments, Plackett-Burman design, and mixture design were performed to fulfill the strain nutritional requirements. Eventually, the optimized formulation contained 19.46 g/L yeast extract; 8.28 g/L whey protein concentrate; 2.26 g/L soy peptone; 30 g/L cerelose; 1 g/L Tween 80; 5 g/L sodium acetate; 0.2 g/L magnesium sulfate and 0.05 g/L manganese sulfate. When L. sakei ACU-2 was cultivated in a bioreactor using the alternative medium, an enhancement of 75.5% of biomass production was achieved, in comparison to its growth in the commercial de Man, Rogosa, and Sharpe medium. Furthermore, a reduction of 62-86% of the cost was also attained. These results support a promising large-scale application of the designed medium for high biomass yields of the starter culture at minor costs.
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