ArticleNature communications2023
Cryo-tomography reveals rigid-body motion and organization of apicomplexan invasion machinery.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 28 citations in OpenAlex.
- The human parasite, Toxoplasma gondii, is paralyzed without two components of the apical polar ring.PLoS pathogens · 2026Article
- The cytoskeleton of pathogenic protists.Bioscience reports · 2026Review
- Two anchoring proteins control daughter apical complex assembly inbioRxiv : the preprint server for biology · 2026Article
- Microtubule inner proteins in apicomplexan parasites.Biochemical Society transactions · 2026Review
- The essential function of the apical polar ring during the blood stage ofbioRxiv : the preprint server for biology · 2026Article
- Transfection of the free-living alga Chromera velia enables direct comparisons with its parasitic apicomplexan relative, Toxoplasma gondii.Journal of cell science · 2026Article
- Atomic models of the Toxoplasma cell invasion machinery.Nature structural & molecular biology · 2026Article
- Evolutionarily convergent mechanism of formin regulation coordinates actin polymerization in apicomplexan parasites.Science advances · 2025Article
- An apical ring protein essential for conoid complex assembly and daughter cell formation in Toxoplasma gondii.Nature communications · 2025Article
- Perforation of the host cell plasma membrane during Toxoplasma invasion requires rhoptry exocytosis.EMBO reports · 2025Article
- DysregulatedLife science alliance · 2025Article
- Transfection of the free-living algabioRxiv : the preprint server for biology · 2025Article
- A practical look at cryo-electron tomography image processing: Key considerations for new biological discoveries.Current opinion in structural biology · 2025Review
- Review
- Cryo-ET reveals the in situ architecture of the polar tube invasion apparatus from microsporidian parasites.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Architecture of theProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The initiation and early development of apical-basal polarity in Toxoplasma gondii.Journal of cell science · 2024Article
- Article
- The initiation and early development of the tubulin-containing cytoskeleton in the human parasiteMolecular biology of the cell · 2024Article
- Sustained rhoptry docking and discharge requires Toxoplasma gondii intraconoidal microtubule-associated proteins.Nature communications · 2024Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
The apical complex is a specialized collection of cytoskeletal and secretory machinery in apicomplexan parasites, which include the pathogens that cause malaria and toxoplasmosis. Its structure and mechanism of motion are poorly understood. We used cryo-FIB-milling and cryo-electron tomography to visualize the 3D-structure of the apical complex in its protruded and retracted states. Averages of conoid-fibers revealed their polarity and unusual nine-protofilament arrangement with associated proteins connecting and likely stabilizing the fibers. Neither the structure of the conoid-fibers nor the architecture of the spiral-shaped conoid complex change during protrusion or retraction. Thus, the conoid moves as a rigid body, and is not spring-like and compressible, as previously suggested. Instead, the apical-polar-rings (APR), previously considered rigid, dilate during conoid protrusion. We identified actin-like filaments connecting the conoid and APR during protrusion, suggesting a role during conoid movements. Furthermore, our data capture the parasites in the act of secretion during conoid protrusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.