ArticleCell host & microbe2023
Characterization of the rotavirus assembly pathway in situ using cryoelectron tomography.
Article in Cell host & microbe, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 41 citations in OpenAlex.
- A novel glycoform of the rotavirus outer capsid protein VP7 is secreted from polarized cells and activates innate immune cells.Journal of virology · 2026Article
- Optical interference for the guidance of cryogenic focused ion beam milling beyond the axial diffraction limit.Nature communications · 2026Article
- TREM-1 Interacts with Rotavirus Proteins and Drives Inflammatory Responses: A Combined Experimental and Computational Approach.Pathogens (Basel, Switzerland) · 2025Article
- Article
- In situ cryo-electron microscopy and tomography of cellular and organismal samples.Current opinion in structural biology · 2025Review
- Structural determinants of rotavirus proteolytic activation.PLoS pathogens · 2025Article
- Article
- TomoCPT: a generalizable model for 3D particle detection and localization in cryo-electron tomograms.Acta crystallographica. Section D, Structural biology · 2025Article
- A single residue switch mediates the broad neutralization of Rotaviruses.Nature communications · 2025Article
- Molecular sociology of virus-induced cellular condensates supporting reovirus assembly and replication.Nature communications · 2024Article
- The Viral K1 Killer Yeast System: Toxicity, Immunity, and Resistance.Yeast (Chichester, England) · 2024Review
- Phase separation and viral factories: unveiling the physical processes supporting RNA packaging in dsRNA viruses.Biochemical Society transactions · 2024Review
- How structural biology has changed our understanding of icosahedral viruses.Journal of virology · 2024Review
- Zooming in and out: Exploring RNA Viral Infections with Multiscale Microscopic Methods.Viruses · 2024Review
- One step at a time: new insights into double-stranded ribonucleic acid virus assembly.Signal transduction and targeted therapy · 2024Article
- The Role of the Host Cytoskeleton in the Formation and Dynamics of Rotavirus Viroplasms.Viruses · 2024Review
- Article
- The rotavirus VP5*/VP8* conformational transition permeabilizes membranes to Ca2.PLoS pathogens · 2024Article
- Sequences at gene segment termini inclusive of untranslated regions and partial open reading frames play a critical role in mammalian orthoreovirus S gene packaging.PLoS pathogens · 2024Article
- Integrative Approaches to Study Virus Structures.Sub-cellular biochemistry · 2024Review
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
Abstract
Rotavirus assembly is a complex process that involves the stepwise acquisition of protein layers in distinct intracellular locations to form the fully assembled particle. Understanding and visualization of the assembly process has been hampered by the inaccessibility of unstable intermediates. We characterize the assembly pathway of group A rotaviruses observed in situ within cryo-preserved infected cells through the use of cryoelectron tomography of cellular lamellae. Our findings demonstrate that the viral polymerase VP1 recruits viral genomes during particle assembly, as revealed by infecting with a conditionally lethal mutant. Additionally, pharmacological inhibition to arrest the transiently enveloped stage uncovered a unique conformation of the VP4 spike. Subtomogram averaging provided atomic models of four intermediate states, including a pre-packaging single-layered intermediate, the double-layered particle, the transiently enveloped double-layered particle, and the fully assembled triple-layered virus particle. In summary, these complementary approaches enable us to elucidate the discrete steps involved in forming an intracellular rotavirus particle.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.