ArticlePloS one2023
Molecular and functional characterization of protease from psychrotrophic Bacillus sp. HM49 in North-western Himalaya.
Article in PloS one, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 23 citations in OpenAlex.
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- Enzymatic Properties and Multifunctional Applications of a Novel Extracellular Cold-Active Alkaline Protease from Stenotrophomonas maltophilia Strain TK-4.Applied biochemistry and biotechnology · 2026Article
- In vitro probiotic properties and anti-Vibrio activity of Bacillus subtilis B13 and its ethyl acetate extract.Antonie van Leeuwenhoek · 2026Article
- Microbial Proteases: Integration into Food Products and Industrial Processes.Current microbiology · 2025Review
- Phenotypic, secondary metabolite, and genomic characterization of Bacillus siamensis B03 from brackish water with Anti‑Vibrio potential.Archives of microbiology · 2025Article
- Genomic and Functional Characterization of Bacillus siamensis B01 with Antimicrobial Activity Against Vibrio spp.Current microbiology · 2025Article
- Proteolytic lactic acid bacteria for food safety and One Health: Degradation of cow's milk allergens and prospects for hypoallergenic dairy development.Veterinary world · 2025Article
- Viral Diversity in Polar Hydrocarbon-Contaminated Soils: A Transect Study from King George Island, Antarctica.Food and environmental virology · 2025Article
- Biochemical features and biotechnological potential of a proteolytic extract from a psychrophilic Antarctic bacterium.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Stable protease from Bacillus licheniformis-MA1 strain: statistical production optimization, kinetic and thermodynamic characterization, and application in silver recovery from used X-ray films.Microbial cell factories · 2025Article
- Comprehensive biochemical, molecular and structural characterization of subtilisin with fibrinolytic potential in bioprocessing.Bioresources and bioprocessing · 2025Article
- Enhanced Mosquito Larvicidal Efficacy and Dehairing Properties of Bacillus thuringiensis Serovar israelensis Strain VCRC-B649 Isolated from Malabar Coast, India.Current microbiology · 2025Article
- Exploring newly isolated thermotolerant, halotolerant and antimicrobial resistant Bacillus subtilis ProNTL1 from tannery waste and its alkaline protease production.Molecular biology reports · 2024Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, a psychrotrophic bacteria producing cold-active protease, was obtained from Dachigam National Park, an ecologically significant habitat in Western Himalayas owing to its varied endemic and endangered flora and fauna. This isolate was identified as Bacillus sp. HM49 via phenotypic, Gram staining, bio-chemical and 16S rRNA gene identification. Isolate HM49 when tested for proteolytic activity revealed prominent hydrolytic zone with the most production at 20 °C and pH, 8.0 post 72 h incubation. This enzyme was purified, enhancing its specific activity to 61.15 U/mg and its characterization studies revealed it to be a cold-alkaline protease being active in a wide pH (6.0-12) and temperature (5-40 °C) range. Amplification of CAASPR gene of HM49 was performed, followed by enzyme-substrate docking studies and MMGBSA providing details about its type, molecular weight validation as well as functional applications. The purified protease of HM49 was tested for laundry applications and the enzyme was found to be compatible with majority of the detergents tested. Its potential as an eco-friendly detergent additive was further validated by wash performance test as it effectively removed recalcitrant blood stains at a low temperature of 20 °C that could be beneficial for fine garments like silk which preferably need cold washing.
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