Evidence map›Paper›PMID 36995668›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Advanced Technologies and Automation in mES Cell Workflow.

Anna Pham, Roger Caothien, Lucinda Tam, Diego Diaz, Marques Jackson, Brian Nakao, Charles Yu, Merone Roose-Girma

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Anna PhamGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Roger CaothienGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Lucinda TamGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Diego DiazGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Marques JacksonGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Brian NakaoGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Charles YuGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA.
Merone Roose-GirmaGenentech, Inc., Department of Molecular Biology, South San Francisco, CA, USA. girma.meron@gene.com.
Genmab (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Gene targeting in mouse ES cells replaces or modifies genes of interest; conditional alleles, reporter knock-ins, and amino acid changes are common examples of how gene targeting is used. To streamline and increase the efficiency in our ES cell pipeline and decrease the timeline for mouse models produced via ES cells, automation is introduced in the pipeline. Below, we describe a novel and effective approach utilizing ddPCR, dPCR, automated DNA purification, MultiMACS, and adenovirus recombinase combined screening workflow that reduces the time between therapeutic target identification and experimental validation.

Indexed as

Embryonic Stem CellsGene TargetingAnimalsAutomationMicePolymerase Chain ReactionWorkflowBiomek i5ddPCR (droplet digital PCR)dPCR (digital PCR)GenotypingMouse embryonic stem cells (ESC)MultiMACS (magnetic-activated cell sorting)

Identifiers

PMID36995668
OpenAlexW4361304843

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.