Evidence map›Paper›PMID 36995665›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Genotyping Genome-Edited Founders and Subsequent Generation.

Matthew Mackenzie, Alex Fower, Alasdair J Allan, Gemma F Codner, Rosie K Bunton-Stasyshyn, Lydia Teboul

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.7field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Matthew MackenzieThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK.
Alex FowerThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK.
Alasdair J AllanThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK.
Gemma F CodnerThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK.
Rosie K Bunton-StasyshynThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK. r.bunton-stasyshyn@har.mrc.ac.uk.
Lydia TeboulThe Mary Lyon Centre, MRC Harwell, Didcot, Oxon, UK. l.teboul@har.mrc.ac.uk.
Mary Lyon Centre at MRC Harwell · GB

Funding

Medical Research Council MC_UP_2201/3
6 · The paper itself

Abstract

Targeted nucleases allow the production of many types of genetic mutations directly in the early embryo. However, the outcome of their activity is a repair event of unpredictable nature, and the founder animals that are produced are generally of a mosaic nature. Here, we present the molecular assays and genotyping strategies that will support the screening of the first generation for potential founders and the validation of positive animals in the subsequent generation, according to the type of mutation generated.

Indexed as

CRISPR-Cas SystemsGene EditingAnimalsGenomeGenotypeMutationAllele QCCRISPR-Cas9Embryo/zygote engineeringGenetic engineeringGenome editingGenotypingMouse modelsTransgenesis

Identifiers

PMID36995665
OpenAlexW4361301427

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.