ArticleMethods in molecular biology (Clifton, N.J.)2023
Highly Efficient Gene Knockout in Medicago truncatula Genotype R108 Using CRISPR-Cas9 System and an Optimized Agrobacterium Transformation Method.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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2 citing papers in PubMed.
- Applying conventional and cell-type-specific CRISPR/Cas9 genome editing in legume plants.aBIOTECH · 2025Review
- An optimised CRISPR Cas9 and Cas12a mutagenesis toolkit for Barley and Wheat.Plant methods · 2024Article
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4 authors.
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Abstract
Medicago truncatula is the model plant species for studying symbioses with nitrogen-fixing rhizobia and arbuscular mycorrhizae, where edited mutants are invaluable for elucidating the contributions of known genes in these processes. Streptococcus pyogenes Cas9 (SpCas9)-based genome editing is a facile means of achieving loss of function, including where multiple gene knockouts are desired in a single generation. We describe how the user can customize our vector to target single or multiple genes, then how the vector is used to make M. truncatula transgenic plants containing target site mutations. Finally, obtaining transgene-free homozygous mutants is covered.
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