Evidence map›Paper›PMID 36995620›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Genetic Engineering of Potato (Solanum tuberosum) Chloroplasts Using the Small Synthetic Plastome "Mini-Synplastome".

Alessandro Occhialini, Alexander C Pfotenhauer, Henry Daniell, C Neal Stewart, Scott C Lenaghan

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Alessandro OcchialiniCenter for Agricultural Synthetic Biology, University of Tennessee Institute of Agriculture, Knoxville, TN, USA.
Alexander C PfotenhauerCenter for Agricultural Synthetic Biology, University of Tennessee Institute of Agriculture, Knoxville, TN, USA.
Henry DaniellDepartment of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, USA.
C Neal StewartCenter for Agricultural Synthetic Biology, University of Tennessee Institute of Agriculture, Knoxville, TN, USA.
Scott C LenaghanDepartment of Food Science, University of Tennessee, Knoxville, TN, USA. slenagha@utk.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the rapidly expanding field of synthetic biology, chloroplasts represent attractive targets for installation of valuable genetic circuits in plant cells. Conventional methods for engineering the chloroplast genome (plastome) have relied on homologous recombination (HR) vectors for site-specific transgene integration for over 30 years. Recently, episomal-replicating vectors have emerged as valuable alternative tools for genetic engineering of chloroplasts. With regard to this technology, in this chapter we describe a method for engineering potato (Solanum tuberosum) chloroplasts to generate transgenic plants using the small synthetic plastome (mini-synplastome). In this method, the mini-synplastome is designed for Golden Gate cloning for easy assembly of chloroplast transgene operons. Mini-synplastomes have the potential to accelerate plant synthetic biology by enabling complex metabolic engineering in plants with similar flexibility of engineered microorganisms.

Indexed as

Solanum tuberosumChloroplastsGenetic EngineeringMetabolic EngineeringPlants, Genetically ModifiedTransgenesChloroplast engineeringChloroplast oriEpisomal replicationMini-synplastomePlastomeSolanum tuberosum

Identifiers

PMID36995620

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.