ArticleMethods in molecular biology (Clifton, N.J.)2023
CRISPR/LbCas12a-Mediated Genome Editing in Soybean.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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Who cites it
6 citing papers in PubMed.
- Enhancing heritable genome editing in soybean by optimizing promoter combinations for the LbCas12a system.BMC plant biology · 2026Article
- Establishment of the CRISPR-Cpf1 gene editing system inSynthetic and systems biotechnology · 2025Article
- A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean.Plant communications · 2024Article
- Versatile plant genome engineering using anti-CRISPR-Cas12a systems.Science China. Life sciences · 2024Article
- Advances in Soybean Genetic Improvement.Plants (Basel, Switzerland) · 2024Review
- Agrobacterial Transformation Enhancement by Improved Competent Cell Preparation and Optimized Electroporation.Life (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
20 authors.
Funding
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Abstract
Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12a nuclease system in soybean. The method uses Agrobacterium-mediated transformation to deliver editing constructs and uses aadA or ALS genes as selectable marker. It only takes about 45 days to obtain greenhouse-ready edited plants at higher than 30% transformation efficiency and 50% editing rate. The method is applicable to other selectable markers including EPSPS and has low transgene chimera rate. The method is also genotype-flexible and has been applied to genome editing of several elite soybean varieties.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.