Evidence map›Paper›PMID 36995599›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Skeletal Muscle Regeneration in Zebrafish.

Tapan G Pipalia, Sami H A Sultan, Jana Koth, Robert D Knight, Simon M Hughes

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.2field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Tapan G PipaliaRandall Centre for Cell & Molecular Biophysics, King's College London, London, UK.
Sami H A SultanCentre for Craniofacial and Regenerative Biology, King's College London, London, UK.
Jana KothRandall Centre for Cell & Molecular Biophysics, King's College London, London, UK.
Robert D KnightCentre for Craniofacial and Regenerative Biology, King's College London, London, UK.
Simon M HughesRandall Centre for Cell & Molecular Biophysics, King's College London, London, UK. s.hughes@kcl.ac.uk.
King's College London · GBJohn Radcliffe Hospital · GB

Funding

Medical Research Council G1001029Medical Research Council MR/N021231/1Medical Research Council MR/W001381/1National Centre for the Replacement, Refinement and Reduction of Animals in Research NC/P002048/1
6 · The paper itself

Abstract

Muscle regeneration models have revealed mechanisms of inflammation, wound clearance, and stem cell-directed repair of damage, thereby informing therapy. Whereas studies of muscle repair are most advanced in rodents, the zebrafish is emerging as an additional model organism with genetic and optical advantages. Various muscle wounding protocols (both chemical and physical) have been published. Here we describe simple, cheap, precise, adaptable, and effective wounding protocols and analysis methods for two stages of a larval zebrafish skeletal muscle regeneration model. We show examples of how muscle damage, ingression of muscle stem cells, immune cells, and regeneration of fibers can be monitored over an extended timecourse in individual larvae. Such analyses have the potential to greatly enhance understanding, by reducing the need to average regeneration responses across individuals subjected to an unavoidably variable wound stimulus.

Indexed as

Satellite Cells, Skeletal MuscleZebrafishAnimalsCell ProliferationMuscle Fibers, SkeletalMuscle, SkeletalStem CellsConfocal live imagingLaser injuryMultiphoton microscopyMuscle precursor cellsMuscle regenerationMuscle woundingNeedlestick injuryPax7Satellite cellSecond harmonic generationSpinning disk imagingZebrafish muscle

Identifiers

PMID36995599
OpenAlexW3157546700

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.