Evidence map›Paper›PMID 36994728›Full record

ArticleArteriosclerosis, thrombosis, and vascular biology2023

ISGylation of NF-κBp65 by SCF

Lian Li, Jiaxing Miao, Nargis Shaheen, Sarah J Taleb, Jian Hu, Qinmao Ye, Jinshan He, Jiasheng Yan, Rama K Mallampalli, Jing Zhao and 1 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Arteriosclerosis, thrombosis, and vascular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. ISGylation: is our genome yearning for such a modification?Acta biochimica et biophysica Sinica · 2025
    Review
  3. SKP1-CUL1-F-box: Key molecular targets affecting disease progression.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025
    Review
  4. Article
  5. Unconventional posttranslational modification in innate immunity.Cellular and molecular life sciences : CMLS · 2024
    Review
  6. Post-translational modifications of p65: state of the art.Frontiers in cell and developmental biology · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 3 countries.

Lian Li *Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Jiaxing Miao *Department of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Nargis ShaheenDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Sarah J TalebDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Jian HuDepartment of Internal Medicine (J. Hu, R.K.M., J.Z., Y.Z.), The Ohio State University, Columbus.
Qinmao YeDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Jinshan HeDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Jiasheng YanDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Rama K MallampalliDepartment of Internal Medicine (J. Hu, R.K.M., J.Z., Y.Z.), The Ohio State University, Columbus.
Jing ZhaoDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.
Yutong ZhaoDepartment of Physiology and Cell Biology, Dorothy M. Davis Heart and Lung Research Institute (L.L., J.M., N.S., S.J.T., Q.Y., J. He, J.Y., J.Z., Y.Z.), The Ohio State University, Columbus.ORCID 0000-0001-6604-9315
Lung Institute · USIHI Corporation (United States) · USThe Ohio State University · USJiaxing University · CN

Funding

Regulation of Cardiolin Byosynthesis in Epithelial InjuryP01HL114453 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MALLAMPALLI, RAMA K · 2014 to 2023
$21.3M
TNF-alpha Control of a Surfactant Enzyme in Lung InjuryR01HL081784 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MALLAMPALLI, RAMA K · 2006 to 2024
$5.4M
Sepsis-Associated Immune Suppression and F-box ProteinsR01HL097376 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI MALLAMPALLI, RAMA K · 2009 to 2025
$5.1M
ISGylation regulates lung endothelial inflammationR01HL157164 · NHLBI · OHIO STATE UNIVERSITY · PI ZHAO, YUTONG · 2021 to 2024
$2.2M
Molecular regulation of anti-inflammatory cytokine receptor in sepsisR01HL136294 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZHAO, YUTONG · 2018 to 2021
$1.8M
Deubiquitinating and inhibiting Hsp90 by USP40 mitigates lung injuryR01HL151513 · NHLBI · OHIO STATE UNIVERSITY · PI ZHAO, JING · 2020 to 2023
$1.7M
Regulation of proteolysis by deubiquiting enzyme in lung inflammatory diseaseR01HL131665 · NHLBI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZHAO, YUTONG · 2017 to 2020
$1.5M
Regulation of Histone Acetyltransferase Stability In SepsisR01GM115389 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI ZHAO, JING · 2015 to 2019
$1.5M
NHLBI NIH HHS P01 HL114453NHLBI NIH HHS R01 HL081784NHLBI NIH HHS R01 HL097376NHLBI NIH HHS R01 HL131665NHLBI NIH HHS R01 HL136294NHLBI NIH HHS R01 HL151513NHLBI NIH HHS R01 HL157164NIGMS NIH HHS R01 GM115389
6 · The paper itself

Abstract

backgroundNF-κB (nuclear factor kappa B) plays a pivotal role in endothelial cell (EC) inflammation. Protein ISGylation is regulated by E3 ISG15 (interferon-stimulated gene 15) ligases; however, ISGylation of NF-κBp65 and its role in EC functions have not been investigated. Here, we investigate whether p65 is ISGylated and the role of its ISGylation in endothelial functions.

methodsIn vitro ISGylation assay and EC inflammation were performed. EC-specific transgenic mice were utilized in a murine model of acute lung injury.

resultsWe find that NF-κBp65 is ISGylated in resting ECs and that the posttranslational modification is reversible. TNFα (tumor necrosis factor alpha) and endotoxin stimulation of EC reduce p65 ISGylation, promoting its serine phosphorylation through reducing its association with a phosphatase WIP1 (wild-type p53-induced phosphatase 1). Mechanistically, an SCF (Skp1-Cul1-F-box) protein E3 ligase SCF

conclusionsTogether, our data reveal a new posttranslational modification of p65 catalyzed by a previously unrecognized role of SCF

Indexed as

Acute Lung InjuryF-Box ProteinsAnimalsCell LineDNA-Binding ProteinsInflammationMiceMice, TransgenicUbiquitin-Protein LigasesDNA-Binding ProteinsF-Box ProteinsFBXL19 protein, mouseUbiquitin-Protein Ligasesacute lung injuryendothelial cellsinflammationphosphorylationprotein processing, posttranslational

Identifiers

PMID36994728
PMCPMC10133096
OpenAlexW4361274266

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.