ArticleJOR spine2023
Harmonization and standardization of nucleus pulposus cell extraction and culture methods.
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Article in JOR spine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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39citing papers in PubMed
13.0field-weighted citation impact, top 1% of its field
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Who cites it
39 citing papers in PubMed, 51 citations in OpenAlex.
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- Restoring disc matrix homeostasis: Dual-miRNA and human platelet lysate as a novel therapeutic strategy.Materials today. Bio · 2026Article
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- Natural photosynthetic system for restoring homeostasis of animal organelle interaction network.Nature communications · 2026Article
- TIE2-positive cells in the nucleus pulposus with a purpose: the who, what and why.Journal of biomedical science · 2026Review
- Influence of the Intervertebral Disc Microenvironment on Matrix Synthesis and Metabolism in Goat Nucleus Pulposus Cells.JOR spine · 2026Article
- Cell-based therapies for intervertebral disc degeneration: Navigating the path from promise to clinical reality.Journal of orthopaedic translation · 2026Review
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- Macrophage Subtypes Distinctly Impact Intervertebral Disc Inflammation and Extracellular Matrix Composition.JOR spine · 2025Article
- Understanding the Physiological Behavior of Disc Cells in an In Vitro Imitation of the Healthy and Degenerated Disc Niche.JOR spine · 2025Article
- Intracellular Detection ofJOR spine · 2025Article
- Advancing Intervertebral Disc Biology via Omics: Implications for Nucleus Pulposus Progenitor Cell-Based Regeneration.JOR spine · 2025Review
- Safety of autologous nucleus pulposus cell transplantation with fibrin glue following endoscopic discectomy in chronic low back pain: a preliminary phase I trial.BMC musculoskeletal disorders · 2025Article
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- Pure platelet-rich plasma delays intervertebral disc degeneration by activating SIRT1-mediated autophagy in nucleus pulposus cells.Journal of orthopaedic surgery and research · 2025Article
- Article
Corrections and comments
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Authors and funding
52 authors at 20 institutions in 9 countries.
Shaghayegh BasatvatBiomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK.ORCID https://orcid.org/0000-0001-6201-4588
Frances C BachDepartment of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands.ORCID https://orcid.org/0000-0002-4481-0051
Marcos N BarcellonaTrinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin The University of Dublin Dublin Ireland.ORCID https://orcid.org/0000-0003-0694-3555
Abbie L BinchBiomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK.
Conor T BuckleyTrinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin The University of Dublin Dublin Ireland.ORCID https://orcid.org/0000-0001-7452-4534
Brian BuenoLeni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.ORCID https://orcid.org/0000-0003-1952-6638
Nadeen O ChahineDepartments of Orthopedic Surgery and Biomedical Engineering Columbia University New York New York USA.ORCID https://orcid.org/0000-0002-0478-6042
Ana CheeDepartment of Orthopedic Surgery Rush University Medical Center Chicago Illinois USA.ORCID https://orcid.org/0000-0002-7977-2737
Laura B CreemersDepartment of Orthopedics University Medical Center Utrecht Utrecht The Netherlands.ORCID https://orcid.org/0000-0002-1585-3052
Stefan DudliCenter for Experimental Rheumatology University of Zurich Zurich Switzerland.ORCID https://orcid.org/0000-0001-7351-713X
Bailey FearingDepartment of Orthopedic Surgery Atrium Health Musculoskeletal Institute Charlotte North Carolina USA.ORCID https://orcid.org/0000-0002-9784-4693
Stephen J FergusonInstitute for Biomechanics ETH Zurich Zurich Switzerland.ORCID https://orcid.org/0000-0003-0650-5889
Jennifer GansauLeni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.ORCID https://orcid.org/0000-0002-1951-2933
Benjamin GantenbeinBone & Joint Program, Department for BioMedical Research (DBMR), Medical Faculty University of Bern Bern Switzerland.ORCID https://orcid.org/0000-0002-9005-0655
Rahul GawriDivision of Orthopaedic Surgery, Department of Surgery McGill University Montreal Canada.ORCID https://orcid.org/0000-0001-9415-1739
Juliane D GlaeserCedars-Sinai Medical Center Los Angeles California USA.
Sibylle GradAO Research Institute Davos Davos Switzerland.ORCID https://orcid.org/0000-0001-9552-3653
Julien GuerreroBone & Joint Program, Department for BioMedical Research (DBMR), Medical Faculty University of Bern Bern Switzerland.ORCID https://orcid.org/0000-0003-4130-4230
Lisbet HaglundDivision of Orthopaedic Surgery, Department of Surgery McGill University Montreal Canada.ORCID https://orcid.org/0000-0002-1288-2149
Paula A HernandezDepartment of Orthopaedic Surgery University of Texas Southwestern Medical Center Dallas Texas USA.ORCID https://orcid.org/0000-0002-8785-7351
Judith A HoylandSchool of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Sciences Centre The University of Manchester Manchester UK.ORCID https://orcid.org/0000-0003-4876-5208
Charles HuangDepartment of Biomedical Engineering University of Miami Coral Gables Florida USA.ORCID https://orcid.org/0000-0002-8848-258X
James C IatridisLeni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.ORCID https://orcid.org/0000-0002-2186-0590
Svenja Illien-JungerDepartment of Orthopaedics Emory University School of Medicine Atlanta GA USA.ORCID https://orcid.org/0000-0001-9895-0693
Liufang JingDepartment of Orthopaedics Emory University School of Medicine Atlanta GA USA.
Petra KrausDepartment of Orthopaedics Emory University School of Medicine Atlanta GA USA.ORCID https://orcid.org/0000-0002-6716-5550
Lisanne T LaaglandDepartment of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands.ORCID https://orcid.org/0000-0002-4456-6167
Gernot LangDepartment of Orthopedics and Trauma Surgery, Medical Center, Faculty of Medicine Albert-Ludwigs-University of Freiburg Freiburg im Breisgau Germany.
Victor LeungDepartment of Orthopaedics & Traumatology The University of Hong Kong Hong Kong SAR China.ORCID https://orcid.org/0000-0002-3406-7225
Zhen LiAO Research Institute Davos Davos Switzerland.ORCID https://orcid.org/0000-0002-9754-6389
Thomas LufkinDepartment of Biology Clarkson University Potsdam New York USA.ORCID https://orcid.org/0000-0002-7902-4771
Josette C van MaanenDepartment of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands.
Emily E McDonnellTrinity Centre for Biomedical Engineering, Trinity Biomedical Sciences Institute, Trinity College Dublin The University of Dublin Dublin Ireland.ORCID https://orcid.org/0000-0002-5064-9591
Chris J PanebiancoLeni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.
Steven M PresciuttiDepartment of Orthopaedics Emory University School of Medicine Atlanta GA USA.ORCID https://orcid.org/0000-0001-6547-9495
Sanjna RaoLeni & Peter W. May Department of Orthopaedics Icahn School of Medicine at Mount Sinai New York New York USA.ORCID https://orcid.org/0000-0002-9784-0262
Stephen M RichardsonSchool of Biological Sciences, Faculty of Biology, Medicine and Health, Manchester Academic Health Sciences Centre The University of Manchester Manchester UK.ORCID https://orcid.org/0000-0002-7637-4135
Sarah RomereimDepartment of Orthopedic Surgery Atrium Health Musculoskeletal Institute Charlotte North Carolina USA.
Tara C SchmitzOrthopaedic Biomechanics, Department of Biomedical Engineering Eindhoven University of Technology Eindhoven The Netherlands.ORCID https://orcid.org/0000-0003-4247-1620
Jordy ScholDepartment of Orthopedic Surgery Tokai University School of Medicine Isehara Japan.ORCID https://orcid.org/0000-0001-5489-2591
Lori SettonDepartments of Biomedical Engineering and Orthopedic Surgery Washington University in St. Louis St. Louis Missouri USA.ORCID https://orcid.org/0000-0001-8857-3871
Dmitriy SheynCedars-Sinai Medical Center Los Angeles California USA.ORCID https://orcid.org/0000-0002-3333-1485
Joseph W SnuggsBiomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK.ORCID https://orcid.org/0000-0002-0200-4596
Y SunDepartment of Orthopaedics & Traumatology The University of Hong Kong Hong Kong SAR China.ORCID https://orcid.org/0000-0002-9728-2205
Xiaohong TanDepartment of Biomedical Engineering Washington University in St. Louis St. Louis Missouri USA.
Marianna A TryfonidouDepartment of Clinical Sciences, Faculty of Veterinary Medicine Utrecht University Utrecht The Netherlands.ORCID https://orcid.org/0000-0002-2333-7162
Nam VoDepartment of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USA.ORCID https://orcid.org/0000-0002-1265-8921
Dong WangDepartment of Orthopaedic Surgery University of Pittsburgh Pittsburgh Pennsylvania USA.ORCID https://orcid.org/0000-0002-8380-3858
Brandon WilliamsDepartment of Orthopedic Surgery Rush University Medical Center Chicago Illinois USA.
Rebecca WilliamsBiomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK.ORCID https://orcid.org/0000-0002-3760-4890
S Tim YoonDepartment of Orthopaedics Emory University School of Medicine Atlanta GA USA.ORCID https://orcid.org/0000-0003-1010-6952
Christine L Le MaitreBiomolecular Sciences Research Centre Sheffield Hallam University Sheffield UK.ORCID https://orcid.org/0000-0003-4489-7107
Emory University · USIcahn School of Medicine at Mount Sinai · USSheffield Hallam University · GBUtrecht University · NLTrinity College Dublin · IEAO Foundation · CHCedars-Sinai Medical Center · USChinese University of Hong Kong · HKManchester Academic Health Science Centre · GBOrthoCarolina · USRush University Medical Center · USUniversity of Bern · CHUniversity of Pittsburgh · USWashington University in St. Louis · USClarkson University · USColumbia University · USEindhoven University of Technology · NLETH Zurich · CHMcGill University · CAMcGill University and Génome Québec Innovation Centre · CA
Funding
Mechanisms for Regenerative Healing in Intervertebral DiscsR01AR080096 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI James C. Iatridis · 2022 to 2026
$3.8MIntervertebral Disc Degeneration and Cross-Talk with the Nervous System - NOSI Diversity SupplementR01AR077678 · NIAMS · WASHINGTON UNIVERSITY · PI SETTON, LORI A., TANG, SIMON YUE-CHEONG · 2020 to 2024
$3.7MInduced pluripotent stem cell therapy for degenerated IVDK01AR071512 · NIAMS · CEDARS-SINAI MEDICAL CENTER · PI SHEYN, DMITRIY · 2018 to 2022
$595kCytoskeletal Regulation of Human Nucleus Pulposus Cell PhenotypeF32AR070579 · NIAMS · WASHINGTON UNIVERSITY · PI FEARING, BAILEY V · 2017 to 2018
$121kModifying High Modulus Hydrogels for Cell Delivery: Intervertebral Disc Repair with Genipin-Crosslinked FibrinF31AR077385 · NIAMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI PANEBIANCO, CHRISTOPHER JAMES · 2021 to 2022
$53kBLRD VA IK2 BX003845NIAMS NIH HHS F31 AR077385NIAMS NIH HHS F32 AR070579NIAMS NIH HHS K01 AR071512NIAMS NIH HHS R01 AR077678NIAMS NIH HHS R01 AR080096
Abstract
Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources. Methods: The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated. Results: Recommended protocols are provided for extraction, expansion, and re-differentiation of NP cells from common species utilized for NP cell culture. Conclusions: This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60-100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide.
Indexed as
cultureharmonizationintervertebralin vitronucleus pulposusstandardization
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.