Evidence map›Paper›PMID 36993525›Full record

ArticlebioRxiv : the preprint server for biology2023

Gain without pain: Adaptation and increased virulence of Zika virus in vertebrate host without fitness cost in mosquito vector.

Anna S Jaeger, Jeffrey Marano, Kasen Riemersma, David Castañeda, Elise Pritchard, Julia Pritchard, Ellie K Bohm, John J Baczenas, Shelby L O'Connor, James Weger-Lucarelli and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Anna S JaegerDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
Jeffrey MaranoDepartment of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University.
Kasen RiemersmaDepartment of Pathobiological Sciences, University of Wisconsin-Madison.
David CastañedaDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
Elise PritchardDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
Julia PritchardDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
Ellie K BohmDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
John J BaczenasDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, United States.
Shelby L O'ConnorDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, United States.
James Weger-LucarelliDepartment of Biomedical Sciences and Pathobiology, Virginia Polytechnic Institute and State University.
Thomas C FriedrichDepartment of Pathobiological Sciences, University of Wisconsin-Madison.
Matthew T AliotaDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities.
University of Minnesota · USUniversity of Wisconsin–Madison · USVirginia Tech · US

Funding

WNPRC Supplemental Request for Nonhuman Primate Enclosures to Equip HIV/AIDS-Related Research FacilitiesP51OD011106 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI Dorota A. Grejner-Brzezinska · 2012 to 2026
$150.7M
ZOLEDRONATE PREVENTS BONE LOSS IN OVARIECTOMIZED RHESUS MONKEYSP51RR000167 · NCRR · UNIVERSITY OF WISCONSIN-MADISON · PI WATKINS, DAVID I · 1985 to 2011
$106.7M
Minnesota Training Program in VirologyT32AI083196 · NIAID · UNIVERSITY OF MINNESOTA · PI Louis M Mansky · 2010 to 2026
$3.2M
Previous exposure to dengue as a risk factor for Zika during pregnancyR01AI132563 · NIAID · UNIVERSITY OF MINNESOTA · PI ALIOTA, MATTHEW T · 2018 to 2022
$2.8M
Zika virus evolutionary dynamics in host adaptationR21AI131454 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI ALIOTA, MATTHEW T · 2017 to 2018
$422k
NCRR NIH HHS P51 RR000167NIAID NIH HHS R01 AI132563NIAID NIH HHS R21 AI131454NIAID NIH HHS T32 AI083196NIH HHS P51 OD011106
6 · The paper itself

Abstract

Zika virus (ZIKV) is now in a post-pandemic period, for which the potential for re-emergence and future spread is unknown. Adding to this uncertainty is the unique capacity of ZIKV to directly transmit between humans via sexual transmission. Recently, we demonstrated that direct transmission of ZIKV between vertebrate hosts leads to rapid adaptation resulting in enhanced virulence in mice and the emergence of three amino acid substitutions (NS2A-A117V, NS2A-A117T, and NS4A-E19G) shared among all vertebrate-passaged lineages. Here, we further characterized these host-adapted viruses and found that vertebrate-passaged viruses also have enhanced transmission potential in mosquitoes. To understand the contribution of genetic changes to the enhanced virulence and transmission phenotype, we engineered these amino acid substitutions, singly and in combination, into a ZIKV infectious clone. We found that NS4A-E19G contributed to the enhanced virulence and mortality phenotype in mice. Further analyses revealed that NS4A-E19G results in increased neurotropism and distinct innate immune signaling patterns in the brain. None of the substitutions contributed to changes in transmission potential in mosquitoes. Together, these findings suggest that direct transmission chains could enable the emergence of more virulent ZIKV strains without compromising mosquito transmission capacity, although the underlying genetics of these adaptations are complex.

Identifiers

PMID36993525
PMCPMC10055270
OpenAlexW4327904988

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.