Evidence map›Paper›PMID 36993356›Full record

ArticlebioRxiv : the preprint server for biology2023

Using DNA flow-stretching assay as a tool to validate the tagging of DNA-binding proteins for single-molecule experiments.

Miranda Molina, Lindsey E Way, Zhongqing Ren, Qin Liao, Xindan Wang, HyeongJun Kim

Open access · greenAbstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Miranda MolinaBiochemistry and Molecular Biology Program, University of Texas Rio Grande Valley, Edinburg, Texas, United States of America.
Lindsey E WayDepartment of Biology, Indiana University, 1001 E 3rd St, Bloomington, Indiana, United States of America.
Zhongqing RenDepartment of Biology, Indiana University, 1001 E 3rd St, Bloomington, Indiana, United States of America.
Qin LiaoDepartment of Biology, Indiana University, 1001 E 3rd St, Bloomington, Indiana, United States of America.
Xindan WangDepartment of Biology, Indiana University, 1001 E 3rd St, Bloomington, Indiana, United States of America.
HyeongJun KimBiochemistry and Molecular Biology Program, University of Texas Rio Grande Valley, Edinburg, Texas, United States of America.
Indiana University Bloomington · USThe University of Texas Rio Grande Valley · US

Funding

Physicochemical properties driving membraneless organelle assembly in bacteriaR01GM143182 · NIGMS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BITEEN, JULIE, MEYER, ANNE SARA · 2021 to 2024
$2.4M
Molecular basis of bacterial chromosome segregation and organizationR35GM143093 · NIGMS · UNIVERSITY OF TEXAS RIO GRANDE VALLEY · PI KIM, HYEONGJUN · 2021 to 2025
$2.0M
How bacterial SMC complexes organize chromosomes (Equipment Supplement)R01GM141242 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI WANG, XINDAN · 2021 to 2025
$1.8M
NIGMS NIH HHS R01 GM141242NIGMS NIH HHS R01 GM143182NIGMS NIH HHS R35 GM143093
6 · The paper itself

Abstract

Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed

Identifiers

PMID36993356
PMCPMC10055205
OpenAlexW4353060553

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.