Evidence map›Paper›PMID 36993168›Full record

ArticlebioRxiv : the preprint server for biology2023

Opposing, spatially-determined epigenetic forces impose restrictions on stochastic olfactory receptor choice.

Elizaveta V Bashkirova, Nell Klimpert, Kevin Monahan, Christine E Campbell, Jason M Osinski, Longzhi Tan, Ira Schieren, Ariel Pourmorady, Beka Stecky, Gilad Barnea and 7 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

17 authors at 5 institutions in 2 countries.

Elizaveta V BashkirovaIntegrated Program in Cellular, Molecular and Biomedical Studies, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, Columbia University, New York, NY, 10032, USA.
Nell KlimpertDepartment of Neuroscience, Division of Biology and Medicine and Robert J. and Nancy D. Carney Institute for Brain Science, Brown University, Providence, RI, USA.
Kevin MonahanDepartment of Biochemistry and Molecular Biology, Rutgers University, NJ, USA.
Christine E CampbellDepartment of Biochemistry, University at Buffalo and New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY, USA.
Jason M OsinskiDepartment of Biochemistry, University at Buffalo and New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY, USA.
Longzhi TanDepartment of Bioengineering, Stanford University, CA, USA.
Ira SchierenZuckerman Mind, Brain, and Behavior Institute, Columbia University, New York, NY, 10027, USA.
Ariel PourmoradyIntegrated Program in Cellular, Molecular and Biomedical Studies, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, Columbia University, New York, NY, 10032, USA.
Beka SteckyZuckerman Mind, Brain, and Behavior Institute, Columbia University, New York, NY, 10027, USA.
Gilad BarneaDepartment of Neuroscience, Division of Biology and Medicine and Robert J. and Nancy D. Carney Institute for Brain Science, Brown University, Providence, RI, USA.
X Sunnie XieBeijing Innovation Center for Genomics, Peking University, Beijing, China.
Ishmail Abdus-SaboorZuckerman Mind, Brain, and Behavior Institute, Columbia University, New York, NY, 10027, USA.
Benjamin ShykindDepartment of Neuroscience, Division of Biology and Medicine and Robert J. and Nancy D. Carney Institute for Brain Science, Brown University, Providence, RI, USA.
Bianca Jones-MarlinZuckerman Mind, Brain, and Behavior Institute, Columbia University, New York, NY, 10027, USA.
Richard M GronostajskiDepartment of Biochemistry, University at Buffalo and New York State Center of Excellence in Bioinformatics and Life Sciences, Buffalo, NY, USA.
Alexander FleischmannDepartment of Neuroscience, Division of Biology and Medicine and Robert J. and Nancy D. Carney Institute for Brain Science, Brown University, Providence, RI, USA.
Stavros LomvardasZuckerman Mind, Brain, and Behavior Institute, Columbia University, New York, NY, 10027, USA.
Columbia University · USAllen Institute for Brain Science · USPeking University · CNRutgers, The State University of New Jersey · USStanford University · US

Funding

The Structure of Olfactory Neural and Perceptual SpacesU19NS112953 · NINDS · NEW YORK UNIVERSITY SCHOOL OF MEDICINE · PI RINBERG, DMITRY · 2019 to 2023
$18.4M
Deciphering nuclear bodies and compartments that govern singular olfactory receptor expression.U01DA040582 · NIDA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI BROWN, LEWIS M., LOMVARDAS, STAVROS · 2015 to 2019
$3.2M
An olfactory subsystem that mediates innate behaviorsR01DC013561 · NIDCD · BROWN UNIVERSITY · PI BARNEA, GILAD · 2014 to 2018
$3.1M
Principles of zonal olfactory receptor gene expressionR01DC018745 · NIDCD · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI LOMVARDAS, STAVROS · 2020 to 2024
$2.3M
Brain Science Compute ClusterS10OD025181 · OD · BROWN UNIVERSITY · PI SANES, JEROME N · 2019 to 2019
$600k
NIDA NIH HHS U01 DA040582NIDCD NIH HHS R01 DC013561NIDCD NIH HHS R01 DC018745NIH HHS S10 OD025181NINDS NIH HHS U19 NS112953
6 · The paper itself

Abstract

Olfactory receptor (OR) choice represents an example of genetically hardwired stochasticity, where every olfactory neuron expresses one out of ~2000 OR alleles in a probabilistic, yet stereotypic fashion. Here, we propose that topographic restrictions in OR expression are established in neuronal progenitors by two opposing forces: polygenic transcription and genomic silencing, both of which are influenced by dorsoventral gradients of transcription factors NFIA, B, and X. Polygenic transcription of OR genes may define spatially constrained OR repertoires, among which one OR allele is selected for singular expression later in development. Heterochromatin assembly and genomic compartmentalization of OR alleles also vary across the axes of the olfactory epithelium and may preferentially eliminate ectopically expressed ORs with more dorsal expression destinations from this "privileged" repertoire. Our experiments identify early transcription as a potential "epigenetic" contributor to future developmental patterning and reveal how two spatially responsive probabilistic processes may act in concert to establish deterministic, precise, and reproducible territories of stochastic gene expression.

Identifiers

PMID36993168
PMCPMC10055043
OpenAlexW4324325357

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.