Evidence map›Paper›PMID 36992917›Full record

ArticleJournal of biological methods2022

Estimating somatic mutation rates by bottlenecked duplex sequencing in non-model organisms:

Eli Sobel, Jeremy E Coate, Sarah Schaack

Abstract read
In one paragraph

Article in Journal of biological methods, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Detection of mutations: from Ames test to duplex sequencing.Frontiers in molecular biosciences · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Eli SobelDepartment of Biology, Reed College, Portland, OR 97202.
Jeremy E CoateDepartment of Biology, Reed College, Portland, OR 97202.
Sarah SchaackDepartment of Biology, Reed College, Portland, OR 97202.

Funding

Tissue, Time, and Genotype: Investigating the Effects of Cellular Environment, Age, and Germline Mutation Rate on the Genome-Wide Rate and Spectrum of Somatic MutationR15GM132861 · NIGMS · REED COLLEGE · PI SCHAACK, SARAH · 2019 to 2019
$408k
NIGMS NIH HHS R15 GM132861
6 · The paper itself

Abstract

Somatic mutations are evolutionarily important as determinants of individual organismal fitness, as well as being a focus of clinical research on age-related disease, such as cancer. Identifying somatic mutations and quantifying mutation rates, however, is extremely challenging and genome-wide somatic mutation rates have only been reported for a few model organisms. Here, we describe the application of Duplex Sequencing on bottlenecked WGS libraries to quantify somatic nuclear genome-wide base substitution rates in

Indexed as

Daphnia magnaDuplex SequencingSingle Molecule Sequencingsomatic mutation

Identifiers

PMID36992917
PMCPMC10040303

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.