ArticleViruses2023
Multiplex RT-qPCR Application in Early Detection of Bovine Respiratory Disease in Healthy Calves.
Article in Viruses, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Development and preliminary evaluation of multiplex PCR assays for the detection of bacterial respiratory pathogens in bovine and ovine.Veterinary and animal science · 2026Article
- Article
- Bovine Respiratory Mycoplasmas and the Commensal-Pathogen Continuum: A Systematic Review of Vaccines and Diagnostic Approaches.Animals : an open access journal from MDPI · 2026Review
- Development and validation of a multiplex TaqMan real-time quantitative PCR assay for the simultaneous detection of six major bacterial respiratory pathogens in goats.Frontiers in veterinary science · 2026Article
- Diagnostic Methods for Bovine Coronavirus: A Review of Recent Advancements and Challenges.Viruses · 2025Review
- Nasal pathobiont abundance is a moderate feedlot-dependent indicator of bovine respiratory disease in beef cattle.Animal microbiome · 2025Article
- Nasal pathobiont abundance does not differ between dairy cattle with or without clinical symptoms of bovine respiratory disease.Animal microbiome · 2025Article
- Multiplex one-step RT‒qPCR assays for simultaneous detection of AMDV, MEV and CDV.BMC veterinary research · 2025Article
- Establishment of a multiplex qPCR assay for the detection of pathogens associated with bovine respiratory disease complex.Frontiers in veterinary science · 2025Article
- Field-validated multiplex RT-qPCR for simultaneous detection of bovine respiratory syncytial virus and bovine parainfluenza virus-3 in bovine respiratory samples.Frontiers in veterinary science · 2025Article
- Schmallenberg virus epidemiology and regional control strategies: diagnostics, vaccines, and vector management.Frontiers in cellular and infection microbiology · 2025Review
- Frequency of Bovine Respiratory Disease Complex Bacterial and Viral Agents Using Multiplex Real-Time qPCR in Quebec, Canada, from 2019 to 2023.Veterinary sciences · 2024Article
- Recent Role of Microorganisms of thePathogens (Basel, Switzerland) · 2024Review
- Establishment and Application of a Triplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay for Differentiation of PEDV, TGEV and PKV.Veterinary sciences · 2024Article
- Advances in Laboratory Diagnosis of Coronavirus Infections in Cattle.Pathogens (Basel, Switzerland) · 2024Review
- Strategies for Bovine Respiratory Disease (BRD) Diagnosis and Prognosis: A Comprehensive Overview.Animals : an open access journal from MDPI · 2024Review
- Specific and sensitive detection of bovine coronavirus using CRISPR-Cas13a combined with RT-RAA technology.Frontiers in veterinary science · 2024Article
- Development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population.Frontiers in chemistry · 2024Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine respiratory diseases (BRD) are associated with various predisposing factors, such as physical and physiological stress factors, and bacterial and viral pathogens. These stressors and viruses suppress immune defenses, leading to bacterial growth in the upper respiratory tract and invasion of pathogens into the lower respiratory tract. Therefore, continuous monitoring of the causative pathogens would contribute to the early detection of BRD. Nasal swabs and sera from 63 clinically healthy calves were continuously collected from seven farms in Iwate prefecture from 2019 to 2021. We attempted to monitor dynamics of BRD-associated pathogens by multiplex real-time RT-PCR (RT-qPCR) using their nasal swab samples. In addition, we attempted to monitor fluctuation of antibody titers against each BRD-associated pathogen by virus neutralization test (VNT) using their sera. In contrast, nasal swabs from 89 calves infected with BRD were collected from 28 farms in Iwate prefecture from 2019 to 2021. We attempted to analyze their nasal swab samples by multiplex RT-qPCR aim to detect BRD-associated pathogens that are dominant in this region. As a result, our analyses using samples from clinically healthy calves showed that positive results by multiplex RT-qPCR were closely related to a significant increase of antibody titers by VNT in bovine coronavirus (BCoV), bovine torovirus (BToV), and bovine respiratory syncytial virus (BRSV). In addition, our data exhibited that BCoV, BToV, BRSV, bovine parainfluenza virus 3, and
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.