Evidence map›Paper›PMID 36987811›Full record

ArticleStem cells (Dayton, Ohio)2023

SATB1 Chromatin Loops Regulate Megakaryocyte/Erythroid Progenitor Expansion by Facilitating HSP70 and GATA1 Induction.

Mark C Wilkes, Hee-Don Chae, Vanessa Scanlon, Alma-Martina Cepika, Ethan P Wentworth, Mallika Saxena, Ascia Eskin, Zugen Chen, Bert Glader, Maria Grazia Roncarolo and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cells (Dayton, Ohio), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Mark C WilkesDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.ORCID 0000-0002-9600-6831
Hee-Don ChaeDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.
Vanessa ScanlonDepartment of Laboratory Medicine, Yale Stem Cell Center, Yale Cooperative Center of Excellence in Hematology, Yale School of Medicine, New Haven, CT, USA.
Alma-Martina CepikaInstitute for Stem Cell Biology and Regenerative Medicine, Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Ethan P WentworthDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.
Mallika SaxenaDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.
Ascia EskinDepartment of Pathology and Laboratory Medicine¸ David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Zugen ChenDepartment of Pathology and Laboratory Medicine¸ David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Bert GladerDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.
Maria Grazia RoncaroloInstitute for Stem Cell Biology and Regenerative Medicine, Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Stanley F NelsonDepartment of Pathology and Laboratory Medicine¸ David Geffen School of Medicine, University of California, Los Angeles, CA, USA.
Kathleen M SakamotoDivision of Hematology/Oncology, Department of Pediatrics, Stanford University, Stanford, CA, USA.
Stanford University · USUniversity of California, Los Angeles · USYale Cancer Center · US

Funding

Training in Pediatric Nonmalignant Hematology and Stem Cell BiologyT32DK098132 · NIDDK · STANFORD UNIVERSITY · PI SAKAMOTO, KATHLEEN M · 2014 to 2022
$1.9M
The Role of Intrinsic and Extrinsic Factors in Megakaryocytic-Erythroid Progenitor Lineage CommitmentK01DK120798 · NIDDK · YALE UNIVERSITY · PI SCANLON, VANESSA M. · 2019 to 2023
$931k
Characterization of the Role of Nemo-like Kinase in Normal and Diamond Blackfan Anemia Models of Erythropoiesis.K01DK123140 · NIDDK · STANFORD UNIVERSITY · PI WILKES, MARK CHRISTOPHER · 2021 to 2025
$763k
NIDDK NIH HHS K01 DK120798NIDDK NIH HHS K01 DK123140NIDDK NIH HHS T32 DK098132
6 · The paper itself

Abstract

Diamond Blackfan anemia (DBA) is an inherited bone marrow failure syndrome associated with severe anemia, congenital malformations, and an increased risk of developing cancer. The chromatin-binding special AT-rich sequence-binding protein-1 (SATB1) is downregulated in megakaryocyte/erythroid progenitors (MEPs) in patients and cell models of DBA, leading to a reduction in MEP expansion. Here we demonstrate that SATB1 expression is required for the upregulation of the critical erythroid factors heat shock protein 70 (HSP70) and GATA1 which accompanies MEP differentiation. SATB1 binding to specific sites surrounding the HSP70 genes promotes chromatin loops that are required for the induction of HSP70, which, in turn, promotes GATA1 induction. This demonstrates that SATB1, although gradually downregulated during myelopoiesis, maintains a biological function in early myeloid progenitors.

Indexed as

Anemia, Diamond-BlackfanMatrix Attachment Region Binding ProteinsCell DifferentiationChromatinGATA1 Transcription FactorHSP70 Heat-Shock ProteinsHumansMegakaryocytesTranscription FactorsChromatinGATA1 protein, humanGATA1 Transcription FactorHSP70 Heat-Shock ProteinsMatrix Attachment Region Binding ProteinsSATB1 protein, humanTranscription FactorschromatinCLOuD9Diamond Blackfan anemiaerythropoiesisGATA1hematopoiesisHSP70megakaryocytesSATB1

Identifiers

PMID36987811
PMCPMC10267687
OpenAlexW4361301582

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.